Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   M2Z51_RS01425 Genome accession   NZ_AP024967
Coordinates   311344..312360 (+) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   Q9KNU6
Organism   Vibrio cholerae strain V130003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 306344..317360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2Z51_RS01410 (V130003_02760) - 306884..307615 (-) 732 WP_000495226.1 glutathione peroxidase -
  M2Z51_RS01415 (V130003_02770) oxyR 307755..308648 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  M2Z51_RS01420 (V130003_02780) - 308724..311201 (-) 2478 WP_032477106.1 penicillin-binding protein 1A -
  M2Z51_RS01425 (V130003_02790) pilM 311344..312360 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  M2Z51_RS01430 (V130003_02800) pilN 312350..312934 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  M2Z51_RS01435 (V130003_02810) pilO 312927..313517 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  M2Z51_RS01440 (V130003_02820) pilP 313507..314025 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  M2Z51_RS01445 (V130003_02830) pilQ 314051..315766 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ family protein Machinery gene
  M2Z51_RS01450 (V130003_02840) aroK 315953..316477 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=76294 M2Z51_RS01425 WP_000520444.1 311344..312360(+) (pilM) [Vibrio cholerae strain V130003]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=76294 M2Z51_RS01425 WP_000520444.1 311344..312360(+) (pilM) [Vibrio cholerae strain V130003]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment