Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OTS06_RS18885 Genome accession   NZ_CP113533
Coordinates   4073749..4074858 (+) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain SHSK47     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4068749..4079858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTS06_RS18850 - 4069373..4069684 (+) 312 WP_024945836.1 hypothetical protein -
  OTS06_RS18855 - 4069733..4070152 (-) 420 WP_168755742.1 DUF4426 domain-containing protein -
  OTS06_RS18860 yggU 4070171..4070470 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  OTS06_RS18865 - 4070470..4071021 (-) 552 WP_016351969.1 YggT family protein -
  OTS06_RS18870 proC 4071062..4071886 (-) 825 WP_270827844.1 pyrroline-5-carboxylate reductase -
  OTS06_RS18875 - 4071945..4072646 (-) 702 WP_029304817.1 YggS family pyridoxal phosphate-dependent enzyme -
  OTS06_RS18880 pilT 4072686..4073720 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  OTS06_RS18885 pilU 4073749..4074858 (+) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  OTS06_RS18890 yaaA 4074890..4075663 (+) 774 WP_106551764.1 peroxide stress protein YaaA -
  OTS06_RS18895 srmB 4075840..4077063 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  OTS06_RS18900 - 4077192..4077902 (+) 711 WP_106551763.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  OTS06_RS18905 brnQ 4078094..4079401 (+) 1308 WP_106553719.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=762822 OTS06_RS18885 WP_017411211.1 4073749..4074858(+) (pilU) [Aeromonas hydrophila strain SHSK47]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=762822 OTS06_RS18885 WP_017411211.1 4073749..4074858(+) (pilU) [Aeromonas hydrophila strain SHSK47]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTTAACGGCCATCTGCAGTCGCTGGCTGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAACGCTATCTGCATACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGTTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGTATAGACACAGCGTCGTTCGACGT
GGCGCTTAAGAGTTCGCTACGCCAGGCTCCGGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTTCCTCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCTCAGCA
ACTGTTGCCCAGCCTGGACGGACGCCGCCGCTGTGCAGCCTTCGAGGTGCTTCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTTGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36