Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   OU800_RS02470 Genome accession   NZ_CP113519
Coordinates   544356..546575 (-) Length   739 a.a.
NCBI ID   WP_268180907.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539356..551575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS02450 (OU800_02450) hslU 539482..540822 (-) 1341 WP_268180901.1 HslU--HslV peptidase ATPase subunit -
  OU800_RS02455 (OU800_02455) hslV 540871..541401 (-) 531 WP_024306625.1 ATP-dependent protease subunit HslV -
  OU800_RS02460 (OU800_02460) - 541797..542477 (-) 681 WP_268180903.1 SPOR domain-containing protein -
  OU800_RS02465 (OU800_02465) argS 542484..544223 (-) 1740 WP_268180905.1 arginine--tRNA ligase -
  OU800_RS02470 (OU800_02470) priA 544356..546575 (-) 2220 WP_268180907.1 primosomal protein N' Machinery gene
  OU800_RS02475 (OU800_02475) rpmE 546736..546948 (+) 213 WP_003459015.1 50S ribosomal protein L31 -
  OU800_RS02480 (OU800_02480) - 546975..547775 (+) 801 WP_268180910.1 thermonuclease family protein -
  OU800_RS02485 (OU800_02485) - 547772..549208 (+) 1437 WP_268180913.1 M48 family metalloprotease -
  OU800_RS02490 (OU800_02490) - 549311..550579 (+) 1269 WP_268180915.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81871.17 Da        Isoelectric Point: 8.4489

>NTDB_id=762667 OU800_RS02470 WP_268180907.1 544356..546575(-) (priA) [Pseudomonas sp. GOM7]
MPDLILRLALPSPLRRLFDYLAPAGVSRSALQPGVRLRVPFGRRELVGILVEVDSQSEVPADKLKPALELLDTRPPLPAT
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAQVRQERYWLAKTGASVDDPRLERAPRQREALKALAQHPHGVAHSLL
SQLQLNRDSLQLLHEKGLVTVELRRNLPHAKPGHWLAQPELPLNAEQRAAVNAVASGWNQFNAYLLAGVTGSGKTEVYLQ
LIHQCLQAGKQALVLIPEINLGPQTFDRFARRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPMKHPGL
IIVDEEHDASYKQQEGLRYHARDLALVRAHQEEVPIVLGSATPSLESLHNAHSGRYALLKLTQRAGGASQPRFLRLDVKS
RPLDSGISGPMQQAIAQTLAAGQQVLVFLNRRGFAPTLLCHDCGWLSECPRCDARMTVHQRYQELRCHHCGHVERQPSHC
PKCQHVDLRPVGAGTERAEERLAVLFPDHPVLRIDRDSTSRKGAMDRLFSTINKGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQLIVQVAGRAGRAEEPGKVIIQSHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFCHL
ALLRAEAHKPGQAESFLDEACSEAEHLLGELGLTGIELLGPVPAPMERRAGRFRAQLLLQGNARAPLHRLLTPWSLALEQ
MPSGRAVRWSLDVDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=762667 OU800_RS02470 WP_268180907.1 544356..546575(-) (priA) [Pseudomonas sp. GOM7]
TTGCCCGACCTGATCCTGCGCCTCGCCCTACCCTCGCCGCTGCGCCGCCTGTTCGACTACCTTGCCCCCGCTGGCGTGTC
GCGCAGCGCCTTGCAGCCAGGTGTACGCCTGCGCGTGCCGTTCGGCCGACGCGAACTGGTCGGCATCCTGGTGGAAGTCG
ACAGCCAGAGCGAGGTACCCGCCGACAAGCTCAAGCCCGCGCTGGAACTGCTCGATACGCGCCCGCCGCTGCCCGCGACG
CTGTTCAAGCTGTGCCTGTGGACGGCGCAGTACTACCAGCACAGCCTCGGCGACACCCTGAGCTGGGCGCTGCCGGTGCT
GCTGCGCCAGGGCGAACCGGCGCAGGTACGTCAGGAACGCTACTGGCTGGCCAAAACGGGCGCCAGCGTCGACGACCCAC
GCCTGGAGCGTGCGCCACGCCAGCGCGAAGCCCTCAAGGCCTTGGCGCAGCATCCGCACGGCGTTGCCCACAGCCTGCTC
AGCCAGTTGCAGCTCAACCGCGACAGCCTGCAACTGCTGCATGAGAAGGGCCTGGTCACGGTCGAACTGCGGCGCAACCT
GCCGCATGCCAAGCCCGGACATTGGCTGGCGCAACCGGAACTGCCGCTCAATGCCGAGCAGCGCGCCGCCGTCAATGCCG
TGGCCTCGGGCTGGAACCAGTTCAACGCCTATCTGCTGGCTGGCGTGACCGGCAGCGGCAAGACCGAGGTCTATCTGCAA
CTGATCCACCAGTGCCTGCAAGCCGGCAAACAGGCCCTGGTGCTGATCCCGGAGATCAACCTCGGCCCGCAGACCTTCGA
CCGCTTCGCCCGGCGCTTCAACGCGCGCATCGCCCTGTTGCACTCGGCGGTGAACGACCGTGACCGCCTCGATGCCTGGT
TGGCGGCGCGCGATGGCGAGGCCGACATCATCATCGGCACCCGCTCGGCGCTGTTCACGCCGATGAAGCACCCAGGGCTG
ATCATCGTCGACGAAGAACACGACGCCTCCTATAAACAGCAGGAAGGCCTGCGCTACCACGCTCGCGACCTGGCGCTGGT
GCGCGCCCATCAGGAAGAGGTGCCCATCGTCCTCGGCTCGGCCACACCTTCGCTGGAAAGCCTGCACAACGCCCATAGCG
GCCGCTACGCCCTGCTCAAGCTGACCCAGCGCGCCGGCGGCGCCAGCCAGCCACGTTTCCTGCGCCTGGACGTGAAGAGC
CGGCCGCTGGACTCGGGCATCTCCGGGCCGATGCAGCAGGCCATCGCCCAGACCCTGGCGGCTGGCCAGCAGGTGCTGGT
GTTCCTCAACCGCCGTGGCTTCGCCCCGACCCTGCTCTGCCACGACTGTGGCTGGCTGTCGGAATGCCCTCGCTGCGATG
CGCGCATGACCGTGCATCAGCGCTACCAGGAACTGCGCTGCCATCACTGCGGTCATGTCGAGCGCCAGCCGAGCCACTGC
CCCAAGTGCCAGCACGTCGACCTGCGCCCGGTAGGAGCCGGCACCGAGCGAGCCGAAGAGCGCCTGGCCGTGCTGTTCCC
CGATCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGAAAAGGCGCGATGGATCGCCTGTTCAGCACCATCAACA
AGGGCGAACCGTGTATCCTGGTCGGCACCCAGATGCTCGCCAAGGGCCACCACTTCCCGCGCGTCACCCTGGTGGCCATT
CTCGATGCCGATGGCGGCCTGTTCTCCGCCGACTTCCGCGCCAGCGAGCGCATGGCCCAGTTGATCGTGCAGGTGGCTGG
CCGCGCCGGGCGCGCCGAAGAGCCCGGCAAGGTGATCATCCAGAGCCACCTGGCCGACCATCCGCTGCTGGTGCAGCTCA
CCGAACAGGGCTACTTCGCCTTCGCCGAACAGGCCCTGAGCGAGCGCCGCGCGGCCGGCCTGCCACCCTTCTGCCACCTG
GCGCTGCTGCGCGCAGAAGCGCATAAACCCGGCCAGGCCGAGAGCTTTCTCGATGAAGCCTGCAGCGAAGCCGAACACCT
GCTGGGCGAGCTGGGCCTGACCGGCATCGAACTGCTCGGCCCGGTGCCTGCGCCGATGGAGCGCCGCGCCGGGCGCTTCC
GCGCGCAACTGCTGCTGCAAGGCAATGCCCGCGCGCCCCTGCACCGCCTGCTCACCCCCTGGTCTCTGGCACTGGAGCAG
ATGCCCAGCGGCCGCGCGGTGCGCTGGTCGCTGGACGTCGACCCGATCGATCTTTTCTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(207-367)

Helicase conserved C-terminal domain (Helicase_C)

(525-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

48.365

99.323

0.48