Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NM686_RS19390 Genome accession   NZ_CP113517
Coordinates   4101211..4102650 (-) Length   479 a.a.
NCBI ID   WP_255189464.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4096211..4107650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS19355 (NM686_019355) - 4096396..4096593 (-) 198 WP_255189457.1 winged helix-turn-helix domain-containing protein -
  NM686_RS19360 (NM686_019360) minE 4096673..4096927 (-) 255 WP_255189458.1 cell division topological specificity factor MinE -
  NM686_RS19365 (NM686_019365) minD 4096927..4097739 (-) 813 WP_255189459.1 septum site-determining protein MinD -
  NM686_RS19370 (NM686_019370) minC 4097761..4098501 (-) 741 WP_255189460.1 septum site-determining protein MinC -
  NM686_RS19375 (NM686_019375) mgtE 4098577..4099935 (-) 1359 WP_255189461.1 magnesium transporter -
  NM686_RS19380 (NM686_019380) rapZ 4099968..4100828 (-) 861 WP_255189462.1 RNase adapter RapZ -
  NM686_RS19385 (NM686_019385) hpf 4100879..4101169 (-) 291 WP_255189463.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NM686_RS19390 (NM686_019390) rpoN/rpoN1 4101211..4102650 (-) 1440 WP_255189464.1 RNA polymerase factor sigma-54 Machinery gene
  NM686_RS19395 (NM686_019395) lptB 4102663..4103388 (-) 726 WP_255189465.1 LPS export ABC transporter ATP-binding protein -
  NM686_RS19400 (NM686_019400) lptA 4103390..4103893 (-) 504 WP_255189466.1 lipopolysaccharide transport periplasmic protein LptA -
  NM686_RS19405 (NM686_019405) lptC 4103880..4104446 (-) 567 WP_255189467.1 LPS export ABC transporter periplasmic protein LptC -
  NM686_RS19410 (NM686_019410) kdsC 4104443..4104976 (-) 534 WP_255189468.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NM686_RS19415 (NM686_019415) - 4104987..4105907 (-) 921 WP_269023008.1 KpsF/GutQ family sugar-phosphate isomerase -
  NM686_RS19420 (NM686_019420) - 4106105..4106923 (+) 819 WP_255189469.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 54021.36 Da        Isoelectric Point: 4.5827

>NTDB_id=762657 NM686_RS19390 WP_255189464.1 4101211..4102650(-) (rpoN/rpoN1) [Methylomonas rapida strain MP1]
MKQSLQLRIGQSLTMTPQLQQAIKLLQMSTLDLQQEIQQALESNMMLEAEEEDLPTLEYREKKTENTDQVTSEGSQTDLP
DELPVDVSWDDVYESMLPVGGDDAADSAEFETFRGKAQTLQDHLIWQLELTHVSERDYAIAVAIIDAINDDGYVAMDLNE
IHQGLLEQLDDVEMDEVRAVLHMVQNFDPVGVAALDLADCLRLQLQQLPDDTPYKADALEFVCRHLELLATCDQNRLMKR
VGFSEAQLKGVLALIRTLDPKPGARLQEADVEYVVPDVFVTKVKDQWQVTLNPDIAPKLRINPFYSGMIKRADNSQDNVS
MKNHLQEARWFIKSLHSRNDTLLRVAKSIVEKQGDFFEHGPIAMKAMVLRDIAEELSLHESTISRVTTQKYMHTPQGVIE
FKYFFSSHVGTDGGGECSATAIRALIKELVGNENPAKPLSDSKISDLLNEKGIQVARRTIAKYREAMSIPSTSQRKRLI

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=762657 NM686_RS19390 WP_255189464.1 4101211..4102650(-) (rpoN/rpoN1) [Methylomonas rapida strain MP1]
ATGAAGCAATCTTTACAACTCAGAATCGGGCAAAGCCTGACAATGACGCCGCAGTTGCAGCAGGCGATCAAGTTGTTGCA
AATGTCGACTTTGGATTTGCAGCAGGAGATCCAGCAGGCGCTCGAGTCCAACATGATGCTGGAAGCGGAAGAAGAGGATT
TGCCGACTCTGGAATATCGGGAAAAGAAAACCGAAAACACCGATCAAGTCACTAGCGAAGGCTCGCAAACCGATTTGCCG
GATGAGTTGCCGGTCGATGTCAGCTGGGACGATGTCTATGAAAGCATGCTGCCGGTTGGTGGCGATGATGCGGCGGACAG
CGCCGAGTTCGAGACTTTTCGCGGCAAGGCGCAAACCTTGCAGGACCATTTGATTTGGCAACTGGAACTAACCCACGTTT
CGGAGCGTGACTACGCGATTGCGGTGGCCATCATCGATGCGATCAATGACGATGGCTATGTTGCCATGGACCTGAACGAG
ATTCATCAGGGTTTATTGGAGCAACTGGACGACGTGGAAATGGATGAGGTGCGGGCAGTGTTGCACATGGTTCAGAATTT
CGATCCGGTGGGGGTTGCCGCTCTGGACTTGGCCGATTGCCTGCGTTTGCAGCTGCAGCAATTACCGGACGATACGCCGT
ATAAGGCAGACGCCTTGGAGTTTGTGTGCAGGCATCTGGAATTATTGGCGACTTGCGATCAAAATCGTTTGATGAAGCGG
GTCGGCTTTAGCGAGGCGCAGTTGAAAGGCGTGCTGGCGCTGATCAGAACGCTAGATCCCAAGCCCGGCGCGCGCTTGCA
GGAAGCCGACGTCGAGTATGTCGTGCCCGACGTCTTCGTCACCAAGGTCAAGGACCAATGGCAAGTGACCTTGAATCCGG
ATATCGCGCCCAAGCTGCGTATCAACCCGTTCTATTCCGGCATGATCAAACGTGCCGATAATAGCCAGGACAATGTCAGC
ATGAAAAACCATCTGCAGGAAGCGCGCTGGTTCATCAAGAGTTTGCACAGTCGCAACGACACCTTGCTGCGGGTCGCGAA
AAGTATCGTCGAAAAACAGGGCGATTTTTTCGAACATGGCCCGATCGCGATGAAGGCCATGGTGCTGAGGGACATTGCCG
AAGAATTGAGTTTGCACGAATCCACGATTTCCCGCGTCACCACGCAAAAATACATGCATACGCCGCAAGGCGTGATCGAA
TTTAAATATTTCTTCTCCAGTCATGTCGGCACGGACGGGGGGGGCGAGTGCTCGGCTACGGCGATAAGGGCACTGATAAA
AGAGTTGGTCGGCAACGAAAATCCGGCCAAACCATTAAGCGATAGTAAAATATCCGACTTATTGAATGAGAAGGGCATCC
AAGTGGCGCGTCGCACCATTGCCAAATATCGCGAAGCCATGTCGATTCCTTCGACCAGCCAGCGGAAAAGGCTAATTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(110-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(321-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.635

100

0.48