Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OWC53_RS02735 Genome accession   NZ_CP113504
Coordinates   581683..583065 (-) Length   460 a.a.
NCBI ID   WP_014914021.1    Uniprot ID   A0A3S0ZXC5
Organism   Pectobacterium brasiliense strain 21PCA_AGRO2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 576683..588065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OWC53_RS02705 (OWC53_02700) pepP 576876..578201 (-) 1326 WP_094370312.1 Xaa-Pro aminopeptidase -
  OWC53_RS02710 (OWC53_02705) - 578259..578846 (-) 588 WP_205592234.1 YecA family protein -
  OWC53_RS02715 (OWC53_02710) zapA 579039..579368 (+) 330 WP_010284667.1 cell division protein ZapA -
  OWC53_RS02725 (OWC53_02720) - 579667..580314 (+) 648 WP_039555513.1 5-formyltetrahydrofolate cyclo-ligase -
  OWC53_RS02730 (OWC53_02725) nadR 580311..581564 (-) 1254 WP_014914020.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  OWC53_RS02735 (OWC53_02730) radA 581683..583065 (-) 1383 WP_014914021.1 DNA repair protein RadA Machinery gene
  OWC53_RS02740 (OWC53_02735) serB 583083..584060 (-) 978 WP_039282511.1 phosphoserine phosphatase -
  OWC53_RS02745 (OWC53_02740) - 584217..584915 (+) 699 WP_040034284.1 YtjB family periplasmic protein -
  OWC53_RS02750 (OWC53_02745) - 584964..585884 (-) 921 WP_268198187.1 hypothetical protein -
  OWC53_RS02755 (OWC53_02750) prfC 586176..587765 (+) 1590 WP_014914025.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49620.19 Da        Isoelectric Point: 7.4218

>NTDB_id=762456 OWC53_RS02735 WP_014914021.1 581683..583065(-) (radA) [Pectobacterium brasiliense strain 21PCA_AGRO2]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=762456 OWC53_RS02735 WP_014914021.1 581683..583065(-) (radA) [Pectobacterium brasiliense strain 21PCA_AGRO2]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTTGAAGCCCTGCCGCGCTTTTCTACCGGC
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGAGTCGTCCCCGGCAGTGCGATTCTGATCGGCGGTAACCCCGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACCGGTGAAGAGTCAC
TACAGCAGGTGGCGATGCGAGCGCATCGTCTCAACCTGCCGACCCAAAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAAGTGATGCATCTCGCGGATAT
TCAATCGTCACCCGGTAGCGTGGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCCAAAACGCGCGGCGTCG
CCATCGTGATGGTCGGCCACGTCACCAAAGACGGTTCGCTCGCCGGGCCAAAAGTATTGGAACACTGCATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGCCATAAAAACCGCTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAACAGGGTTTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGTTCGGTCATGGTGGTATGGGAAGGTACGCGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTGGATCAA
TCGATGATGGCTAACCCGCGTCGCGTGGCGGTCGGGCTGGAGCAAAATCGCCTGGCGATCCTGCTGGCGGTATTACATCG
CCATGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTGCTCTCGCTGGTCTCCAGCTTCCGCGATCGCCCACTGCCGCAGGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGCGAAATCCGCCCGGTTCCTAGCGGCCAAGAGCGAATTACCGAAGCCGCCAAGCATGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTCTTCGGCGTGAAAAAGCTGGCCGATGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0ZXC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426