Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G6L90_RS00045 Genome accession   NZ_CP124967
Coordinates   7784..9334 (-) Length   516 a.a.
NCBI ID   WP_060722825.1    Uniprot ID   A0A822V1P6
Organism   Agrobacterium tumefaciens strain O54/95     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2784..14334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6L90_RS00015 (G6L90_00015) rdgB 2996..3640 (-) 645 WP_003503799.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  G6L90_RS00020 (G6L90_00020) rph 3658..4374 (-) 717 WP_060722824.1 ribonuclease PH -
  G6L90_RS00025 (G6L90_00025) hrcA 4510..5601 (+) 1092 WP_003503796.1 heat-inducible transcriptional repressor HrcA -
  G6L90_RS00030 (G6L90_00030) grpE 5716..6351 (+) 636 WP_003503794.1 nucleotide exchange factor GrpE -
  G6L90_RS00035 (G6L90_00035) ptsN 6433..6894 (-) 462 WP_003503792.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G6L90_RS00040 (G6L90_00040) raiA 6977..7549 (-) 573 WP_003503790.1 ribosome-associated translation inhibitor RaiA -
  G6L90_RS00045 (G6L90_00045) rpoN/rpoN1 7784..9334 (-) 1551 WP_060722825.1 RNA polymerase factor sigma-54 Machinery gene
  G6L90_RS00050 (G6L90_00050) lptB 9600..10409 (-) 810 WP_003503786.1 LPS export ABC transporter ATP-binding protein -
  G6L90_RS00055 (G6L90_00055) - 10419..10979 (-) 561 WP_170318979.1 LptA/OstA family protein -
  G6L90_RS00060 (G6L90_00060) lptC 10996..11661 (-) 666 WP_111791904.1 LPS export ABC transporter periplasmic protein LptC -
  G6L90_RS00065 (G6L90_00065) sppA 11896..12849 (+) 954 WP_111791903.1 signal peptide peptidase SppA -
  G6L90_RS00070 (G6L90_00070) - 12876..13178 (+) 303 WP_003493505.1 integration host factor subunit beta -
  G6L90_RS00075 (G6L90_00075) - 13245..13586 (+) 342 WP_060722827.1 LapA family protein -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 56799.97 Da        Isoelectric Point: 4.4686

>NTDB_id=761736 G6L90_RS00045 WP_060722825.1 7784..9334(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain O54/95]
MALSASLLLRQNQSLVMTPQLMQSIQLLQMTHFELTQFIAQEVERNPLLEIAANDGDLSSDAPVEAESFGDADSEGSERS
ATVTSDSDDWYGDRAANLGEQLDTSFENVFPDDAEPRKADAPELAGQWKSMPGQESGESYDLDDFVAARQSLGDHLNQQL
PLAISAAEDRMIADALIGQLDETGYIAADAVDDVAERLGAAPSAVEHVLKTLQGFDPPGVFSRSLSECLAIQLAQKDRLD
PAMRAFVDNLELLAKRDFASLKKICGVDEEDLLDMLAEIRTLNPRPGAGYDSMVSETIVPDIIVRPSSTGGWLVEINPET
LPRVLINQSYFAEVSKHKARAGEDQDFLAECMQTAHWLTRSLDQRARTIMKVATEIVRQQDAFLVNGVDQLRPLNLKTVA
DAIKMHESTVSRVTSKKYMLTPRGLFELKYFFSVSISAVEGGDSHSAEAVRHRIKAMIAQEAAEAVLSDDDIVDNLKKSG
IDIARRTVAKYREAMNIPSSVQRRREKKAQAKLSAF

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=761736 G6L90_RS00045 WP_060722825.1 7784..9334(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain O54/95]
ATGGCATTGTCTGCCAGCCTTTTGCTGCGTCAAAACCAGTCTCTTGTGATGACACCGCAACTGATGCAGTCCATCCAGCT
GCTTCAGATGACGCATTTCGAGCTGACGCAGTTCATCGCGCAGGAGGTGGAGCGCAATCCATTGCTGGAAATTGCGGCAA
ACGATGGCGATTTGAGCAGCGATGCCCCGGTAGAGGCGGAGAGTTTCGGCGACGCGGACAGCGAAGGCTCGGAGAGATCC
GCCACGGTGACATCAGACAGTGACGATTGGTATGGCGATCGCGCCGCAAATCTCGGCGAGCAACTGGATACCAGCTTCGA
AAACGTCTTTCCCGACGATGCTGAGCCCCGAAAAGCCGATGCGCCTGAACTGGCCGGCCAATGGAAATCCATGCCCGGTC
AGGAGTCCGGGGAAAGTTATGACCTCGATGATTTCGTCGCGGCCAGACAGAGCCTCGGCGACCATCTCAATCAGCAATTG
CCGCTCGCCATATCAGCCGCCGAGGACCGGATGATCGCCGATGCGCTGATCGGCCAGCTCGATGAAACCGGCTATATTGC
GGCTGATGCCGTCGATGATGTCGCCGAACGGCTGGGAGCGGCGCCGTCGGCAGTCGAGCACGTCCTGAAGACGCTTCAGG
GCTTCGACCCACCGGGTGTATTTTCCCGTTCCCTGAGCGAATGTCTGGCGATCCAGCTGGCCCAGAAGGATCGGCTAGAC
CCGGCCATGCGGGCTTTCGTCGATAATCTGGAACTTCTGGCGAAACGCGACTTCGCCTCCTTGAAAAAAATCTGCGGCGT
CGATGAGGAAGACCTGCTCGATATGCTGGCGGAAATCCGCACGCTCAACCCCCGCCCCGGCGCAGGTTACGACTCGATGG
TTTCGGAGACCATCGTTCCCGACATTATCGTTCGCCCCTCCTCCACCGGCGGCTGGCTGGTGGAAATCAACCCCGAGACC
CTGCCGCGCGTGCTCATCAACCAGAGTTACTTCGCCGAGGTATCGAAACATAAGGCGCGCGCGGGCGAGGATCAGGATTT
TCTGGCCGAATGCATGCAGACGGCCCATTGGCTGACCCGCAGCCTAGACCAGCGCGCCCGGACAATCATGAAGGTGGCGA
CGGAAATCGTGCGCCAGCAGGATGCCTTCCTCGTCAATGGTGTCGACCAGCTACGCCCGCTGAACCTCAAGACGGTGGCC
GACGCCATCAAGATGCACGAATCCACCGTCAGCCGGGTGACGTCCAAAAAATACATGCTGACGCCACGCGGTCTGTTTGA
GCTCAAATATTTCTTCAGCGTCTCGATCAGCGCCGTGGAAGGCGGAGACAGCCATTCGGCGGAAGCGGTGCGTCACCGCA
TCAAGGCCATGATCGCGCAGGAGGCCGCCGAGGCAGTCCTTTCCGACGACGACATCGTCGACAATCTGAAAAAGAGCGGC
ATCGATATCGCCCGCCGCACGGTCGCCAAATATCGCGAGGCGATGAACATCCCCTCCTCCGTGCAGAGGCGTCGGGAAAA
GAAGGCGCAGGCCAAGCTTTCCGCCTTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-505)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A822V1P6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

35.283

100

0.362