Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G6L16_RS00045 Genome accession   NZ_CP124963
Coordinates   7685..9235 (-) Length   516 a.a.
NCBI ID   WP_174001272.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain AF1/95     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2685..14235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6L16_RS00015 (G6L16_000015) rdgB 2843..3487 (-) 645 WP_003514751.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  G6L16_RS00020 (G6L16_000020) rph 3505..4221 (-) 717 WP_003514752.1 ribonuclease PH -
  G6L16_RS00025 (G6L16_000025) hrcA 4358..5449 (+) 1092 WP_097101329.1 heat-inducible transcriptional repressor HrcA -
  G6L16_RS00030 (G6L16_000030) grpE 5563..6198 (+) 636 WP_097101331.1 nucleotide exchange factor GrpE -
  G6L16_RS00035 (G6L16_000035) ptsN 6325..6786 (-) 462 WP_003493488.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G6L16_RS00040 (G6L16_000040) hpf 6869..7450 (-) 582 WP_097101333.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G6L16_RS00045 (G6L16_000045) rpoN/rpoN1 7685..9235 (-) 1551 WP_174001272.1 RNA polymerase factor sigma-54 Machinery gene
  G6L16_RS00050 (G6L16_000050) lptB 9389..10198 (-) 810 WP_410171779.1 LPS export ABC transporter ATP-binding protein -
  G6L16_RS00055 (G6L16_000055) - 10208..10774 (-) 567 WP_097101335.1 LptA/OstA family protein -
  G6L16_RS00060 (G6L16_000060) lptC 10788..11453 (-) 666 WP_003514764.1 LPS export ABC transporter periplasmic protein LptC -
  G6L16_RS00065 (G6L16_000065) sppA 11703..12656 (+) 954 WP_174001273.1 signal peptide peptidase SppA -
  G6L16_RS00070 (G6L16_000070) - 12683..12985 (+) 303 WP_003493505.1 integration host factor subunit beta -
  G6L16_RS00075 (G6L16_000075) - 13051..13392 (+) 342 WP_003514766.1 LapA family protein -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 57154.31 Da        Isoelectric Point: 4.4268

>NTDB_id=761700 G6L16_RS00045 WP_174001272.1 7685..9235(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain AF1/95]
MALSASLLLRQNQSLVMTPQLMQSIQLLQMTHFELTQFIAQEVERNPLLEIAANDGDLSNDTPVDAENFGDGDSEGSDKS
APVTADSDDWYGDRAANLGEQLDTSFENVFPDDTEPRKPDAPELAGQWKSMPGQESGESYDLDDFVAAKQTLSDHLNQQL
PLAISSVEDRMIADALIGQLDETGYIAVDAIEDVAERLGTAPSAVERILETLQGFDPPGVFSRSLSECLAIQLAQKDRLD
PAMRAFIDNLELLAKRDFASLKKLCGVDEEDLLDMLAEIRTLNPRPGAGYDSTVSETIVPDIIIRPSSAGGWLVEINPET
LPRVLINQTYFAEVSRHKSREGEDQDFLSECMQTAHWLTRSLDQRARTIMKVATEIVRQQDAFLVNGVDHLRPLNLKTVA
DAIKMHESTVSRVTSKKYMLTPRGLFELKYFFSVSISSVEGGDSHSAEAVRHRIKAMIAQEAADAVLSDDDIVDNLKKSG
IDLARRTVAKYREAMNIPSSVQRRREKKALARLSAF

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=761700 G6L16_RS00045 WP_174001272.1 7685..9235(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain AF1/95]
ATGGCATTATCTGCCAGCCTTTTGCTGCGTCAAAACCAGTCTCTCGTGATGACACCGCAACTGATGCAGTCCATCCAGCT
GCTTCAGATGACGCATTTCGAACTGACGCAGTTCATCGCGCAGGAGGTGGAGCGCAATCCTCTGCTTGAAATTGCGGCAA
ACGACGGCGATTTGAGCAACGATACCCCTGTTGATGCGGAGAATTTCGGCGATGGCGACAGCGAAGGGTCCGACAAGTCC
GCGCCGGTAACAGCCGACAGCGACGACTGGTACGGCGACCGCGCGGCAAATCTCGGCGAGCAGCTCGACACCAGCTTCGA
AAATGTCTTTCCCGACGATACCGAACCCAGAAAGCCCGACGCACCCGAACTGGCGGGGCAGTGGAAATCCATGCCCGGGC
AGGAGTCCGGCGAAAGTTACGATCTCGACGACTTCGTTGCCGCGAAACAGACCCTCAGCGACCATCTCAATCAGCAATTG
CCTCTCGCCATATCCTCTGTGGAAGACAGGATGATCGCTGATGCGCTGATCGGCCAGCTCGACGAGACCGGATATATCGC
CGTAGATGCCATCGAAGACGTCGCTGAACGGCTTGGGACGGCGCCTTCGGCAGTGGAGCGCATTTTGGAGACGCTTCAGG
GCTTTGATCCTCCCGGCGTGTTTTCCCGCTCGCTGAGCGAATGTCTGGCGATCCAGCTTGCCCAGAAGGACAGGCTTGAT
CCGGCAATGCGGGCTTTTATCGACAATCTGGAGCTTCTGGCGAAACGGGATTTCGCTTCTCTAAAAAAGCTCTGCGGCGT
GGATGAGGAAGACCTTCTCGACATGCTGGCGGAAATCCGCACGCTCAATCCGCGTCCCGGCGCTGGTTACGATTCGACCG
TTTCCGAGACAATCGTTCCTGATATCATCATTCGCCCTTCCTCTGCCGGCGGCTGGCTTGTGGAAATCAACCCCGAGACC
CTGCCGCGCGTGCTCATCAACCAGACCTATTTCGCAGAGGTTTCAAGGCACAAGTCGCGTGAGGGAGAGGATCAGGATTT
CCTTTCCGAATGCATGCAGACGGCCCATTGGCTGACCCGCAGCCTCGACCAGCGCGCCAGAACGATCATGAAGGTGGCGA
CCGAAATCGTGCGCCAGCAGGACGCCTTTCTCGTCAATGGCGTGGACCACCTGCGCCCGCTGAACCTCAAGACGGTGGCC
GACGCCATCAAGATGCACGAATCCACCGTCAGCCGGGTGACGTCGAAAAAATACATGCTGACGCCGCGCGGCCTGTTCGA
GCTGAAATATTTCTTCAGTGTTTCCATCAGTTCGGTGGAAGGCGGTGACAGCCATTCGGCAGAAGCGGTGCGTCATCGCA
TCAAGGCGATGATCGCGCAGGAGGCCGCCGACGCAGTGCTTTCCGATGACGATATCGTCGATAATCTGAAAAAGAGTGGT
ATCGATCTTGCCCGCCGCACCGTGGCCAAATATCGCGAGGCGATGAACATCCCATCCTCCGTTCAGAGACGGCGCGAGAA
GAAGGCCCTGGCCAGACTGTCGGCGTTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-330)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-51)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

36.243

100

0.37