Detailed information NTG0761578

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: OR983_RS31900
• Genome accession: NZ_CP113278
• Coordinates: 3161588..3162748 (+)
• Length: 386 a.a.
• NCBI ID: WP_011657978.1
• Uniprot ID: Q0BBP8
• Organism: Burkholderia ambifaria strain HSJ1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3156588..3167748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OR983_RS31870 (OR983_31870)	-	3157202..3157810 (+)	609	WP_006753282.1	50S ribosomal protein L25/general stress protein Ctc	-
OR983_RS31875 (OR983_31875)	pth	3157940..3158539 (+)	600	WP_006760396.1	aminoacyl-tRNA hydrolase	-
OR983_RS31880 (OR983_31880)	hisC	3158665..3159732 (+)	1068	WP_011657975.1	histidinol-phosphate transaminase	-
OR983_RS31885 (OR983_31885)	-	3159837..3160103 (-)	267	WP_011657976.1	YfhL family 4Fe-4S dicluster ferredoxin	-
OR983_RS31890 (OR983_31890)	coaD	3160163..3160660 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
OR983_RS31895 (OR983_31895)	rsmD	3160772..3161386 (-)	615	WP_041491281.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
OR983_RS31900 (OR983_31900)	pilA	3161588..3162748 (+)	1161	WP_011657978.1	signal recognition particle-docking protein FtsY	Machinery gene
OR983_RS31905 (OR983_31905)	maiA	3162858..3163502 (-)	645	WP_041491400.1	maleylacetoacetate isomerase	-
OR983_RS31910 (OR983_31910)	ybiB	3163692..3164666 (+)	975	WP_041491401.1	DNA-binding protein YbiB	-
OR983_RS31915 (OR983_31915)	leuA	3165057..3166703 (+)	1647	WP_011657981.1	2-isopropylmalate synthase	-
OR983_RS31920 (OR983_31920)	-	3166847..3167230 (-)	384	WP_011657982.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41170.99 Da
• Isoelectric Point: 4.8019

>NTDB_id=761578 OR983_RS31900 WP_011657978.1 3161588..3162748(+) (pilA) [Burkholderia ambifaria strain HSJ1]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAADARQAIEAPPAPAQPQPAAPAVVMTVTPTNDGSDDVVEAVEIVP
PPTQDASAKKSWLTRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALFD

Nucleotide

• Length: 1161 bp

>NTDB_id=761578 OR983_RS31900 WP_011657978.1 3161588..3162748(+) (pilA) [Burkholderia ambifaria strain HSJ1]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGATGCGCCCGCCGCCGATGCCCGTCAGGCGATCGAAGCCCCGCCAGCCCCCGCGCAACCGCAACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCCGCGAAGAAATCGTGGCTCACCCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
CACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAAGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCGACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGATCTGCTCGTCGAACTGCTGAAGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAATCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGATGCGCTCGGCCTCACGGGCCTGATCGTCACGAAGCTCGACGGCACCGCCAAGGGCGG
GATTCTGGCGGCGATCGCGCGGCAGCGCCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAACGCCGTGGAATTCGCGGACGCGCTATTCGACTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: Q0BBP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.632	100	0.57