Detailed information NTG0761563

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: OR984_RS31905
• Genome accession: NZ_CP113275
• Coordinates: 3161708..3162868 (+)
• Length: 386 a.a.
• NCBI ID: WP_011657978.1
• Uniprot ID: Q0BBP8
• Organism: Burkholderia ambifaria strain HSJ1v
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3156708..3167868

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OR984_RS31875 (OR984_31825)	-	3157322..3157930 (+)	609	WP_006753282.1	50S ribosomal protein L25/general stress protein Ctc	-
OR984_RS31880 (OR984_31830)	pth	3158060..3158659 (+)	600	WP_006760396.1	aminoacyl-tRNA hydrolase	-
OR984_RS31885 (OR984_31835)	hisC	3158785..3159852 (+)	1068	WP_011657975.1	histidinol-phosphate transaminase	-
OR984_RS31890 (OR984_31840)	-	3159957..3160223 (-)	267	WP_011657976.1	YfhL family 4Fe-4S dicluster ferredoxin	-
OR984_RS31895 (OR984_31845)	coaD	3160283..3160780 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
OR984_RS31900 (OR984_31850)	rsmD	3160892..3161506 (-)	615	WP_041491281.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
OR984_RS31905 (OR984_31855)	pilA	3161708..3162868 (+)	1161	WP_011657978.1	signal recognition particle-docking protein FtsY	Machinery gene
OR984_RS31910 (OR984_31860)	maiA	3162978..3163622 (-)	645	WP_041491400.1	maleylacetoacetate isomerase	-
OR984_RS31915 (OR984_31865)	ybiB	3163812..3164786 (+)	975	WP_041491401.1	DNA-binding protein YbiB	-
OR984_RS31920 (OR984_31870)	leuA	3165177..3166823 (+)	1647	WP_011657981.1	2-isopropylmalate synthase	-
OR984_RS31925 (OR984_31875)	-	3166967..3167350 (-)	384	WP_011657982.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 41170.99 Da
• Isoelectric Point: 4.8019

>NTDB_id=761563 OR984_RS31905 WP_011657978.1 3161708..3162868(+) (pilA) [Burkholderia ambifaria strain HSJ1v]
MFSFFKRFKKTQESDSTESQSADALQPDEPSDAPAADARQAIEAPPAPAQPQPAAPAVVMTVTPTNDGSDDVVEAVEIVP
PPTQDASAKKSWLTRLKTGLAKTGSSITGVFVNTKIDEDLYEELEAALLMSDAGVDATEYLLGALREKVKTGRLTDPQQV
KSALHDLLVELLKPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVISKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALFD

Nucleotide

• Length: 1161 bp

>NTDB_id=761563 OR984_RS31905 WP_011657978.1 3161708..3162868(+) (pilA) [Burkholderia ambifaria strain HSJ1v]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGTCCGACTCCACGGAATCGCAATCGGCCGACGCGCTGCAACC
GGACGAGCCGTCCGATGCGCCCGCCGCCGATGCCCGTCAGGCGATCGAAGCCCCGCCAGCCCCCGCGCAACCGCAACCGG
CCGCGCCGGCCGTCGTGATGACGGTCACGCCGACCAACGACGGCAGCGACGACGTCGTCGAAGCGGTCGAGATCGTGCCG
CCGCCGACGCAGGACGCCTCCGCGAAGAAATCGTGGCTCACCCGCCTGAAAACGGGGCTCGCGAAGACGGGTTCGAGCAT
CACCGGCGTGTTCGTCAACACGAAGATCGACGAGGATCTGTACGAAGAACTCGAAGCCGCGCTGCTGATGTCCGACGCCG
GCGTCGATGCGACCGAGTACCTGCTCGGTGCGCTGCGCGAGAAAGTGAAAACGGGCCGGCTGACCGATCCGCAGCAGGTG
AAGAGCGCGCTGCACGATCTGCTCGTCGAACTGCTGAAGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACGGGCGTGAACGGCGCGGGCAAGACCACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCG
ACCAATCGGTGCTGCTGGCCGCGGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTCCAGCAGGAAAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGGTGAAGC
GCGTGATCTCGAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGATGCGCTCGGCCTCACGGGCCTGATCGTCACGAAGCTCGACGGCACCGCCAAGGGCGG
GATTCTGGCGGCGATCGCGCGGCAGCGCCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAACGCCGTGGAATTCGCGGACGCGCTATTCGACTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-385)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: Q0BBP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.632	100	0.57