Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ORP33_RS12305 Genome accession   NZ_CP113256
Coordinates   2404813..2405850 (-) Length   345 a.a.
NCBI ID   WP_029726718.1    Uniprot ID   -
Organism   Bacillus subtilis strain K1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2399813..2410850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORP33_RS12255 (ORP33_12015) tasA 2399911..2400696 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  ORP33_RS12260 (ORP33_12020) sipW 2400760..2401332 (-) 573 WP_003230181.1 signal peptidase I SipW -
  ORP33_RS12265 (ORP33_12025) tapA 2401316..2402077 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  ORP33_RS12270 (ORP33_12030) yqzG 2402349..2402675 (+) 327 WP_029317915.1 YqzG/YhdC family protein -
  ORP33_RS12275 (ORP33_12035) spoIITA 2402717..2402896 (-) 180 WP_213414620.1 YqzE family protein -
  ORP33_RS12280 (ORP33_12040) comGG 2402967..2403341 (-) 375 WP_225722048.1 ComG operon protein ComGG Machinery gene
  ORP33_RS12285 (ORP33_12045) comGF 2403342..2403725 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  ORP33_RS12290 (ORP33_12050) comGE 2403751..2404098 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  ORP33_RS12295 (ORP33_12055) comGD 2404082..2404513 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  ORP33_RS12300 (ORP33_12060) comGC 2404503..2404799 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  ORP33_RS12305 (ORP33_12065) comGB 2404813..2405850 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  ORP33_RS12310 (ORP33_12070) comGA 2405837..2406907 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  ORP33_RS12315 (ORP33_12075) - 2407120..2407317 (-) 198 WP_029726717.1 hypothetical protein -
  ORP33_RS12320 (ORP33_12080) corA 2407319..2408272 (-) 954 WP_004399136.1 magnesium transporter CorA -
  ORP33_RS12325 (ORP33_12085) yqhB 2408415..2409743 (+) 1329 WP_029726716.1 hemolysin family protein -
  ORP33_RS12330 (ORP33_12090) rsbRD 2409796..2410632 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39702.10 Da        Isoelectric Point: 9.6257

>NTDB_id=761266 ORP33_RS12305 WP_029726718.1 2404813..2405850(-) (comGB) [Bacillus subtilis strain K1]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDILFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=761266 ORP33_RS12305 WP_029726718.1 2404813..2405850(-) (comGB) [Bacillus subtilis strain K1]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATACTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.381

93.623

0.93