Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OSV60_RS16020 Genome accession   NZ_CP113077
Coordinates   3396524..3397324 (-) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 86II/2C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3391524..3402324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSV60_RS16000 (OSV60_16000) - 3392589..3393287 (-) 699 WP_000058788.1 YfgM family protein -
  OSV60_RS16005 (OSV60_16005) hisS 3393316..3394608 (-) 1293 WP_098713587.1 histidine--tRNA ligase -
  OSV60_RS16010 (OSV60_16010) ispG 3394605..3395720 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OSV60_RS16015 (OSV60_16015) - 3395742..3396533 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  OSV60_RS16020 (OSV60_16020) pilF 3396524..3397324 (-) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OSV60_RS16025 (OSV60_16025) rlmN 3397340..3398572 (-) 1233 WP_032043679.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OSV60_RS16030 (OSV60_16030) ndk 3398695..3399126 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  OSV60_RS16035 (OSV60_16035) iscX 3399240..3399440 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  OSV60_RS16040 (OSV60_16040) - 3399540..3400091 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  OSV60_RS16045 (OSV60_16045) - 3400116..3401414 (-) 1299 WP_085940557.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=759972 OSV60_RS16020 WP_002027454.1 3396524..3397324(-) (pilF) [Acinetobacter baumannii strain 86II/2C]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=759972 OSV60_RS16020 WP_002027454.1 3396524..3397324(-) (pilF) [Acinetobacter baumannii strain 86II/2C]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726