Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OSH15_RS16640 Genome accession   NZ_CP113074
Coordinates   3454203..3455003 (-) Length   266 a.a.
NCBI ID   WP_179994561.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3449203..3460003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSH15_RS16620 (OSH15_16635) - 3450268..3450966 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  OSH15_RS16625 (OSH15_16640) hisS 3450995..3452287 (-) 1293 WP_000095263.1 histidine--tRNA ligase -
  OSH15_RS16630 (OSH15_16645) ispG 3452284..3453399 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OSH15_RS16635 (OSH15_16650) - 3453421..3454212 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  OSH15_RS16640 (OSH15_16655) pilF 3454203..3455003 (-) 801 WP_179994561.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OSH15_RS16645 (OSH15_16660) rlmN 3455019..3456251 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OSH15_RS16650 (OSH15_16665) ndk 3456374..3456805 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  OSH15_RS16655 (OSH15_16670) iscX 3456919..3457119 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  OSH15_RS16660 (OSH15_16675) - 3457219..3457770 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  OSH15_RS16665 (OSH15_16680) - 3457795..3459093 (-) 1299 WP_104159583.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30075.39 Da        Isoelectric Point: 9.0211

>NTDB_id=759930 OSH15_RS16640 WP_179994561.1 3454203..3455003(-) (pilF) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=759930 OSH15_RS16640 WP_179994561.1 3454203..3455003(-) (pilF) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACATAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729