Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H7R23_RS16945 Genome accession   NZ_AP021936
Coordinates   3528933..3529733 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain WP2-W18-ESBL-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523933..3534733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R23_RS16925 (WP2W18E11_33120) - 3524998..3525696 (-) 699 WP_017398816.1 YfgM family protein -
  H7R23_RS16930 (WP2W18E11_33130) hisS 3525725..3527017 (-) 1293 WP_032005186.1 histidine--tRNA ligase -
  H7R23_RS16935 (WP2W18E11_33140) ispG 3527014..3528129 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H7R23_RS16940 (WP2W18E11_33150) - 3528151..3528942 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  H7R23_RS16945 (WP2W18E11_33160) pilF 3528933..3529733 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H7R23_RS16950 (WP2W18E11_33170) rlmN 3529748..3530983 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H7R23_RS16955 (WP2W18E11_33180) ndk 3531107..3531538 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  H7R23_RS16960 (WP2W18E11_33190) iscX 3531652..3531852 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  H7R23_RS16965 (WP2W18E11_33200) - 3531952..3532503 (-) 552 WP_017482038.1 phosphatase PAP2 family protein -
  H7R23_RS16970 (WP2W18E11_33210) - 3532528..3533817 (-) 1290 WP_182918112.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=75987 H7R23_RS16945 WP_002114699.1 3528933..3529733(-) (pilF) [Acinetobacter pittii strain WP2-W18-ESBL-11]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=75987 H7R23_RS16945 WP_002114699.1 3528933..3529733(-) (pilF) [Acinetobacter pittii strain WP2-W18-ESBL-11]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744

Multiple sequence alignment