Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   OS082_RS01320 Genome accession   NZ_CP113007
Coordinates   289425..291053 (+) Length   542 a.a.
NCBI ID   WP_001186596.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Ryze     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 288058..338172 289425..291053 within 0


Gene organization within MGE regions


Location: 288058..338172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OS082_RS01315 ccrA 288058..289404 (+) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene
  OS082_RS01320 ccrB 289425..291053 (+) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  OS082_RS01325 - 291524..291874 (+) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  OS082_RS01330 - 291959..292270 (+) 312 WP_000833212.1 DUF960 family protein -
  OS082_RS01335 - 292289..292810 (+) 522 WP_000836008.1 DUF1643 domain-containing protein -
  OS082_RS01340 - 292812..293018 (+) 207 WP_001077283.1 hypothetical protein -
  OS082_RS01345 - 293011..293334 (+) 324 WP_000088091.1 JAB domain-containing protein -
  OS082_RS01350 - 293529..295421 (+) 1893 WP_014532403.1 site-specific integrase -
  OS082_RS01355 - 295428..295805 (+) 378 WP_001041908.1 DUF6262 family protein -
  OS082_RS01360 - 295991..296356 (+) 366 WP_000159127.1 ArsR/SmtB family transcription factor -
  OS082_RS01365 - 296349..298763 (+) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  OS082_RS01370 - 298844..299461 (+) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  OS082_RS01375 - 299735..300373 (-) 639 Protein_272 sulfite exporter TauE/SafE family protein -
  OS082_RS01380 cstR 300373..300633 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  OS082_RS01385 - 300769..301833 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  OS082_RS01390 cstB 301865..303199 (+) 1335 Protein_275 persulfide dioxygenase-sulfurtransferase CstB -
  OS082_RS01395 - 303313..304461 (-) 1149 WP_000427807.1 ROK family transcriptional regulator -
  OS082_RS01400 psm-mec 304781..304849 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  OS082_RS01405 mecI 304934..305305 (-) 372 Protein_278 mecA-type methicillin resistance repressor MecI -
  OS082_RS01410 mecR1 305305..307062 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  OS082_RS01415 mecA 307159..309168 (+) 2010 WP_267790023.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  OS082_RS01420 - 309214..309642 (-) 429 WP_000872606.1 MaoC family dehydratase -
  OS082_RS01425 - 309739..310482 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  OS082_RS01430 - 311560..311949 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  OS082_RS01435 - 311985..312659 (+) 675 WP_001105988.1 IS6-like element IS257 family transposase -
  OS082_RS01440 - 312778..313293 (+) 516 WP_000377078.1 hypothetical protein -
  OS082_RS01445 merB 313488..314138 (-) 651 WP_000790333.1 organomercurial lyase MerB -
  OS082_RS01450 merA 314220..315863 (-) 1644 WP_000193219.1 mercury(II) reductase -
  OS082_RS01455 merT 315921..316307 (-) 387 WP_001794630.1 mercuric transport protein MerT -
  OS082_RS01460 - 316395..317075 (-) 681 WP_000010352.1 cytochrome c biogenesis CcdA family protein -
  OS082_RS01465 - 317072..317425 (-) 354 WP_000746201.1 TlpA family protein disulfide reductase -
  OS082_RS01470 merR 317442..317849 (-) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  OS082_RS01475 - 318149..319495 (-) 1347 WP_267810092.1 dihydrolipoyl dehydrogenase family protein -
  OS082_RS01480 - 319778..320452 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  OS082_RS01485 - 320514..322727 (+) 2214 Protein_294 type I restriction endonuclease subunit R -
  OS082_RS01490 - 322807..323373 (-) 567 WP_000759939.1 PepSY domain-containing protein -
  OS082_RS01495 - 323523..323744 (-) 222 WP_001070883.1 hypothetical protein -
  OS082_RS01500 - 323758..323922 (-) 165 WP_001803222.1 hypothetical protein -
  OS082_RS01505 - 324211..324627 (-) 417 WP_000941331.1 hypothetical protein -
  OS082_RS01510 - 324690..325217 (-) 528 WP_000717928.1 hypothetical protein -
  OS082_RS01515 - 325352..325840 (-) 489 WP_000397419.1 hypothetical protein -
  OS082_RS01520 - 325856..326146 (-) 291 WP_000065262.1 hypothetical protein -
  OS082_RS01525 - 326203..327075 (-) 873 WP_000373151.1 HTH domain-containing protein -
  OS082_RS01530 - 327245..327391 (-) 147 Protein_303 JAB domain-containing protein -
  OS082_RS01535 - 327623..328285 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  OS082_RS15700 - 328677..328778 (+) 102 WP_418896055.1 erythromycin resistance leader peptide -
  OS082_RS01540 erm(A) 328792..329523 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  OS082_RS01545 - 329649..330431 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  OS082_RS01550 - 330582..330959 (-) 378 WP_000361059.1 DUF6262 family protein -
  OS082_RS01555 - 330966..332858 (-) 1893 Protein_309 site-specific integrase -
  OS082_RS01560 - 332855..333940 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  OS082_RS01565 - 334059..334376 (-) 318 WP_023180038.1 JAB domain-containing protein -
  OS082_RS01570 - 334397..334903 (-) 507 WP_000848380.1 DUF1643 domain-containing protein -
  OS082_RS01575 - 334919..335230 (-) 312 WP_001166716.1 DUF960 family protein -
  OS082_RS01580 - 335324..335662 (-) 339 WP_000175892.1 SAUGI family uracil-DNA glycosylase inhibitor -
  OS082_RS01585 tnpA 335954..336439 (-) 486 WP_267810093.1 IS200/IS605 family transposase -
  OS082_RS01590 ccrC 336487..338172 (-) 1686 WP_000676600.1 cassette chromosome recombinase CcrC -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=758957 OS082_RS01320 WP_001186596.1 289425..291053(+) (ccrB) [Staphylococcus aureus strain Ryze]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPELQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=758957 OS082_RS01320 WP_001186596.1 289425..291053(+) (ccrB) [Staphylococcus aureus strain Ryze]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAAAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865