Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P7I85_RS24550 Genome accession   NZ_CP124622
Coordinates   5253934..5255427 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain 2021CK-01159     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5248934..5260427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I85_RS24520 (P7I85_24520) - 5249146..5249955 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  P7I85_RS24525 (P7I85_24525) kdsD 5250369..5251349 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  P7I85_RS24530 (P7I85_24530) - 5251349..5251888 (+) 540 WP_282414021.1 HAD family hydrolase -
  P7I85_RS24535 (P7I85_24535) lptC 5251897..5252469 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  P7I85_RS24540 (P7I85_24540) lptA 5252456..5252983 (+) 528 WP_282414022.1 lipopolysaccharide transport periplasmic protein LptA -
  P7I85_RS24545 (P7I85_24545) lptB 5252983..5253708 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  P7I85_RS24550 (P7I85_24550) rpoN/rpoN1 5253934..5255427 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  P7I85_RS24555 (P7I85_24555) raiA 5255505..5255813 (+) 309 WP_003094359.1 ribosome-associated translation inhibitor RaiA -
  P7I85_RS24560 (P7I85_24560) ptsN 5255827..5256291 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  P7I85_RS24565 (P7I85_24565) rapZ 5256293..5257153 (+) 861 WP_003094361.1 RNase adapter RapZ -
  P7I85_RS24570 (P7I85_24570) - 5257183..5257455 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  P7I85_RS24575 (P7I85_24575) - 5257552..5258484 (-) 933 WP_019485801.1 ZIP family metal transporter -
  P7I85_RS24580 (P7I85_24580) - 5258503..5259114 (-) 612 WP_003098862.1 superoxide dismutase -
  P7I85_RS24585 (P7I85_24585) - 5259127..5259576 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=758792 P7I85_RS24550 WP_003094357.1 5253934..5255427(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2021CK-01159]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=758792 P7I85_RS24550 WP_003094357.1 5253934..5255427(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2021CK-01159]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGGATCGGCGCTCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGATAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477