Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OSB35_RS25970 Genome accession   NZ_CP111139
Coordinates   3007245..3008753 (+) Length   502 a.a.
NCBI ID   WP_004195216.1    Uniprot ID   A0AAX1X7H0
Organism   Burkholderia pseudomallei strain MSHR1713     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3002245..3013753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSB35_RS25945 (OSB35_25945) kdsD 3003477..3004460 (+) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -
  OSB35_RS25950 (OSB35_25950) - 3004460..3004999 (+) 540 WP_004200146.1 KdsC family phosphatase -
  OSB35_RS25955 (OSB35_25955) lptC 3005005..3005619 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  OSB35_RS25960 (OSB35_25960) lptA 3005638..3006303 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  OSB35_RS25965 (OSB35_25965) lptB 3006300..3007076 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  OSB35_RS25970 (OSB35_25970) rpoN/rpoN1 3007245..3008753 (+) 1509 WP_004195216.1 RNA polymerase factor sigma-54 Machinery gene
  OSB35_RS25975 (OSB35_25975) hpf 3008903..3009262 (+) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  OSB35_RS25980 (OSB35_25980) ptsN 3009495..3010004 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OSB35_RS25985 (OSB35_25985) hprK 3010133..3011101 (+) 969 WP_076856869.1 HPr(Ser) kinase/phosphatase -
  OSB35_RS25990 (OSB35_25990) rapZ 3011187..3012080 (+) 894 WP_004195227.1 RNase adapter RapZ -
  OSB35_RS25995 (OSB35_25995) - 3012107..3012739 (+) 633 WP_004522856.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54889.04 Da        Isoelectric Point: 4.8740

>NTDB_id=758057 OSB35_RS25970 WP_004195216.1 3007245..3008753(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain MSHR1713]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=758057 OSB35_RS25970 WP_004195216.1 3007245..3008753(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain MSHR1713]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691