Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   OQE53_RS12015 Genome accession   NZ_CP111072
Coordinates   2487696..2488310 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain YFI-E109     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2482696..2493310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQE53_RS11985 rpsT 2482999..2483265 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  OQE53_RS11990 holA 2483282..2484323 (-) 1042 Protein_2316 DNA polymerase III subunit delta -
  OQE53_RS11995 - 2484363..2484514 (-) 152 Protein_2317 hypothetical protein -
  OQE53_RS12000 - 2484555..2484689 (+) 135 WP_003152870.1 YqzM family protein -
  OQE53_RS12005 comEC 2484708..2487059 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  OQE53_RS12010 - 2487060..2487629 (-) 570 WP_003152868.1 ComE operon protein 2 -
  OQE53_RS12015 comEA 2487696..2488310 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  OQE53_RS12020 comER 2488369..2489190 (+) 822 WP_012118027.1 late competence protein ComER -
  OQE53_RS12025 - 2489259..2489996 (-) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  OQE53_RS12030 rsfS 2489993..2490349 (-) 357 WP_007408260.1 ribosome silencing factor -
  OQE53_RS12035 yqeK 2490353..2490928 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  OQE53_RS12040 - 2490918..2491487 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  OQE53_RS12045 yhbY 2491498..2491788 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  OQE53_RS12050 aroE 2491782..2492624 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=757596 OQE53_RS12015 WP_032873955.1 2487696..2488310(-) (comEA) [Bacillus velezensis strain YFI-E109]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=757596 OQE53_RS12015 WP_032873955.1 2487696..2488310(-) (comEA) [Bacillus velezensis strain YFI-E109]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363