Detailed information NTG0757474

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA54_RS02150
• Genome accession: NZ_CP111032
• Coordinates: 454333..455481 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PALA54
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 449333..460481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA54_RS02125	-	450484..450780 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA54_RS02130 (PALA54_00415)	-	450777..451370 (-)	594	WP_003084544.1	YggT family protein	-
PALA54_RS02135 (PALA54_00416)	proC	451381..452202 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA54_RS02140 (PALA54_00417)	-	452214..452906 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA54_RS02145 (PALA54_00418)	pilT	453121..454155 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA54_RS02150 (PALA54_00419)	pilU	454333..455481 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PALA54_RS02155 (PALA54_00420)	czcD	455489..456388 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA54_RS02160 (PALA54_00421)	-	456543..456947 (+)	405	WP_003084560.1	NINE protein	-
PALA54_RS02165 (PALA54_00422)	-	457190..458563 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA54_RS02170 (PALA54_00423)	-	458560..459744 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=757474 PALA54_RS02150 WP_003110016.1 454333..455481(+) (pilU) [Pseudomonas aeruginosa strain PALA54]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=757474 PALA54_RS02150 WP_003110016.1 454333..455481(+) (pilU) [Pseudomonas aeruginosa strain PALA54]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382