Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OLG56_RS03715 Genome accession   NZ_CP111029
Coordinates   774579..775559 (+) Length   326 a.a.
NCBI ID   WP_061377599.1    Uniprot ID   A0A5H5K427
Organism   Salmonella sp. 2018103     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769579..780559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLG56_RS03685 (OLG56_03685) - 770157..771164 (+) 1008 WP_207011547.1 DUF1202 domain-containing protein -
  OLG56_RS03690 (OLG56_03690) hemW 771255..772391 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  OLG56_RS03695 (OLG56_03695) - 772384..772977 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  OLG56_RS03700 (OLG56_03700) yggU 772985..773275 (-) 291 WP_061377600.1 DUF167 family protein YggU -
  OLG56_RS03705 (OLG56_03705) - 773272..773838 (-) 567 WP_001094848.1 YggT family protein -
  OLG56_RS03710 (OLG56_03710) - 773857..774561 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  OLG56_RS03715 (OLG56_03715) pilT 774579..775559 (+) 981 WP_061377599.1 type IV pilus twitching motility protein PilT Machinery gene
  OLG56_RS03720 (OLG56_03720) - 775691..776377 (+) 687 WP_001607997.1 IclR family transcriptional regulator -
  OLG56_RS03725 (OLG56_03725) ruvX 776424..776840 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  OLG56_RS03730 (OLG56_03730) - 776840..777403 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  OLG56_RS03735 (OLG56_03735) gshB 777619..778566 (-) 948 WP_061377598.1 glutathione synthase -
  OLG56_RS03740 (OLG56_03740) rsmE 778586..779317 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OLG56_RS03745 (OLG56_03745) endA 779394..780101 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36108.34 Da        Isoelectric Point: 7.5355

>NTDB_id=757419 OLG56_RS03715 WP_061377599.1 774579..775559(+) (pilT) [Salmonella sp. 2018103]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRAHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=757419 OLG56_RS03715 WP_061377599.1 774579..775559(+) (pilT) [Salmonella sp. 2018103]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAGCGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H5K427

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.036

100

0.368
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362