Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OEI98_RS12525 Genome accession   NZ_CP110888
Coordinates   2358302..2359663 (-) Length   453 a.a.
NCBI ID   WP_003870692.1    Uniprot ID   M8CUC3
Organism   Thermoanaerobacter sp. RKWS2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2353302..2364663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEI98_RS12495 (OEI98_002499) ispF 2353929..2354426 (-) 498 WP_004404878.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  OEI98_RS12505 (OEI98_002501) - 2354419..2355661 (-) 1243 Protein_2380 lipase family alpha/beta hydrolase -
  OEI98_RS12510 (OEI98_002502) ispD 2355728..2356411 (-) 684 WP_006569578.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  OEI98_RS12515 (OEI98_002503) - 2356494..2357597 (-) 1104 WP_266038191.1 PIN/TRAM domain-containing protein -
  OEI98_RS12520 (OEI98_002504) - 2357880..2358275 (+) 396 WP_003870691.1 hypothetical protein -
  OEI98_RS12525 (OEI98_002505) radA 2358302..2359663 (-) 1362 WP_003870692.1 DNA repair protein RadA Machinery gene
  OEI98_RS12530 (OEI98_002506) - 2359710..2360975 (-) 1266 WP_003870693.1 Nramp family divalent metal transporter -
  OEI98_RS12535 (OEI98_002507) - 2361071..2361535 (-) 465 WP_003870694.1 metal-dependent transcriptional regulator -
  OEI98_RS12540 (OEI98_002508) - 2361608..2364058 (-) 2451 WP_003870696.1 ATP-dependent Clp protease ATP-binding subunit -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50972.81 Da        Isoelectric Point: 5.3387

>NTDB_id=756842 OEI98_RS12525 WP_003870692.1 2358302..2359663(-) (radA) [Thermoanaerobacter sp. RKWS2]
MAKSTTKFVCRECGFESSKWMGRCPNCDSWNSFEEETIEDSKNFRKNKDRSQIFLLEDVTFSEEERIKTGIEEFDRVLGG
GIVKGSLVLIGGDPGIGKSTLLLQVAGNISDNRKVLYVSGEESIQQIKLRADRILKEKQQIYLLCETNIEEIERKIDENK
PDFVIIDSIQTMYTEESETIPGSVSQVRQVTQRLMEIAKQKDISIFLIGHVTKEGAIAGPKVLEHMVDTVLYFEGDRTQS
FRILRAVKNRFGATHEIGVFDMEEDGLCEISNPSEFILSNKPKDVSGTAVVCSIQGTRPILMEVQSLICKTTFGVPRRMA
TGFDYNRFILLLAVIEKRLGINLSQFDAYVNVAGGMKIQEPAADLGIIASSLSGYFNIPIPEDVCFIGEVGLTGEVRGVS
NIDKRIGEAQKMGFSQVFIPEMKTKEIKKFENIKIIKVRDIKEIMDFLRKIKQ

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=756842 OEI98_RS12525 WP_003870692.1 2358302..2359663(-) (radA) [Thermoanaerobacter sp. RKWS2]
ATGGCCAAATCAACTACAAAATTTGTGTGTAGAGAGTGTGGCTTTGAAAGCAGTAAATGGATGGGAAGATGTCCTAATTG
TGACAGCTGGAATTCTTTTGAGGAAGAAACGATAGAGGATTCAAAAAATTTTAGGAAAAATAAAGATAGATCGCAAATAT
TTCTTCTCGAAGATGTAACTTTTTCAGAAGAAGAAAGAATAAAGACAGGGATTGAAGAGTTTGACAGGGTTTTAGGTGGC
GGAATTGTAAAAGGTTCTCTTGTTTTAATAGGTGGTGACCCAGGAATTGGGAAGTCTACTTTGCTATTACAAGTAGCAGG
TAATATTTCTGATAACAGAAAGGTACTTTATGTGTCAGGAGAGGAATCAATTCAACAGATTAAACTGAGGGCAGATAGAA
TTTTGAAAGAAAAGCAACAAATATATCTCTTGTGCGAAACAAATATAGAAGAGATAGAGAGAAAAATTGATGAGAATAAG
CCGGATTTTGTTATTATTGATTCTATACAGACAATGTACACTGAGGAAAGTGAGACAATACCAGGAAGTGTGTCTCAGGT
AAGGCAAGTGACTCAAAGGCTGATGGAGATTGCAAAACAAAAAGACATAAGTATTTTTTTGATAGGACATGTTACAAAAG
AAGGGGCAATAGCAGGCCCTAAAGTTTTGGAACATATGGTAGACACAGTTTTGTATTTTGAAGGGGATAGGACCCAGTCT
TTTAGGATATTGCGGGCTGTAAAAAACCGTTTTGGCGCAACTCACGAAATAGGAGTTTTTGATATGGAGGAGGATGGGCT
TTGTGAGATAAGCAATCCTTCTGAATTTATTCTCTCAAATAAGCCGAAAGATGTTTCAGGGACTGCTGTTGTATGTTCTA
TTCAAGGGACGAGGCCAATTTTAATGGAAGTGCAGTCTTTGATATGTAAGACTACTTTTGGAGTGCCGCGAAGAATGGCC
ACTGGATTTGACTACAATCGTTTTATATTGCTTTTGGCAGTTATAGAAAAAAGACTGGGCATAAATCTTTCACAATTTGA
TGCATATGTAAATGTGGCTGGAGGAATGAAAATTCAAGAGCCAGCTGCTGACCTTGGAATAATTGCTTCATCTCTTTCTG
GATATTTTAATATACCTATTCCTGAAGATGTTTGTTTTATAGGAGAGGTGGGATTGACGGGAGAAGTGAGAGGAGTCAGC
AATATAGATAAACGAATAGGTGAAGCACAAAAGATGGGATTTTCTCAAGTTTTTATTCCTGAGATGAAAACAAAAGAAAT
AAAAAAGTTTGAGAATATAAAAATTATTAAGGTAAGAGATATAAAAGAAATTATGGATTTTTTAAGAAAGATAAAACAAT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-215)

Subunit ChlI of Mg-chelatase (ChlI)

(340-421)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M8CUC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.319

99.779

0.532
  radA Streptococcus mitis NCTC 12261

51.562

98.896

0.51
  radA Streptococcus pneumoniae Rx1

51.562

98.896

0.51
  radA Streptococcus pneumoniae D39

51.562

98.896

0.51
  radA Streptococcus pneumoniae R6

51.562

98.896

0.51
  radA Streptococcus pneumoniae TIGR4

51.562

98.896

0.51
  radA Streptococcus mitis SK321

51.562

98.896

0.51