Detailed information    

insolico Bioinformatically predicted

Overview


Name   rok   Type   Regulator
Locus tag   OPU65_RS04080 Genome accession   NZ_CP110812
Coordinates   802653..803129 (-) Length   158 a.a.
NCBI ID   WP_059231482.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CamBx3     
Function   repression of comK (predicted from homology)   
Competence regulation

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 800374..851723 802653..803129 within 0


Gene organization within MGE regions


Location: 800374..851723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPU65_RS04070 (OPU65_04070) - 800374..800958 (-) 585 WP_243940516.1 TetR family transcriptional regulator -
  OPU65_RS04075 (OPU65_04075) - 801396..802016 (+) 621 WP_265626942.1 hypothetical protein -
  OPU65_RS04080 (OPU65_04080) rok 802653..803129 (-) 477 WP_059231482.1 hypothetical protein Regulator
  OPU65_RS04085 (OPU65_04085) - 803266..803865 (-) 600 WP_265626943.1 GNAT family N-acetyltransferase -
  OPU65_RS04090 (OPU65_04090) - 803898..804370 (-) 473 Protein_815 DUF4944 domain-containing protein -
  OPU65_RS04095 (OPU65_04095) - 804476..805075 (-) 600 WP_265626944.1 cyclopropane-fatty-acyl-phospholipid synthase family protein -
  OPU65_RS04100 (OPU65_04100) - 805251..805730 (-) 480 WP_265626945.1 hypothetical protein -
  OPU65_RS04105 (OPU65_04105) - 805782..806024 (-) 243 WP_265626946.1 hypothetical protein -
  OPU65_RS04110 (OPU65_04110) - 806084..807064 (-) 981 WP_265626947.1 S9 family peptidase -
  OPU65_RS04115 (OPU65_04115) - 807243..807536 (-) 294 WP_243940506.1 hypothetical protein -
  OPU65_RS04120 (OPU65_04120) - 808174..808602 (-) 429 WP_265626948.1 HIT family protein -
  OPU65_RS04125 (OPU65_04125) - 809634..809837 (-) 204 WP_243940500.1 hypothetical protein -
  OPU65_RS04130 (OPU65_04130) - 810441..811169 (-) 729 WP_265626949.1 UPF0489 family protein -
  OPU65_RS04135 (OPU65_04135) - 811402..813123 (+) 1722 WP_265626950.1 T7SS effector LXG polymorphic toxin -
  OPU65_RS04140 (OPU65_04140) - 813142..813486 (+) 345 WP_265626951.1 hypothetical protein -
  OPU65_RS23185 - 813562..813855 (-) 294 Protein_826 peptidoglycan-binding domain-containing protein -
  OPU65_RS23190 - 813928..814641 (-) 714 Protein_827 N-acetylmuramoyl-L-alanine amidase -
  OPU65_RS04155 (OPU65_04155) - 814693..814956 (-) 264 WP_265626952.1 phage holin -
  OPU65_RS04160 (OPU65_04160) - 814972..815241 (-) 270 WP_265626953.1 hemolysin XhlA family protein -
  OPU65_RS04165 (OPU65_04165) - 815304..815480 (-) 177 WP_006637259.1 XkdX family protein -
  OPU65_RS04170 (OPU65_04170) - 815477..816664 (-) 1188 WP_265626954.1 phage baseplate upper protein -
  OPU65_RS04175 (OPU65_04175) - 816678..819323 (-) 2646 WP_265626955.1 peptidase G2 autoproteolytic cleavage domain-containing protein -
  OPU65_RS04180 (OPU65_04180) - 819359..821068 (-) 1710 WP_265626956.1 phage tail protein -
  OPU65_RS04185 (OPU65_04185) - 821081..821917 (-) 837 WP_265626957.1 phage tail family protein -
  OPU65_RS04190 (OPU65_04190) - 821917..825810 (-) 3894 WP_265626958.1 phage tail tape measure protein -
  OPU65_RS04195 (OPU65_04195) - 825823..826005 (-) 183 WP_265627930.1 hypothetical protein -
  OPU65_RS04200 (OPU65_04200) gpG 826008..826346 (-) 339 WP_265626959.1 phage tail assembly chaperone G -
  OPU65_RS04205 (OPU65_04205) - 826406..827014 (-) 609 WP_265626960.1 major tail protein -
  OPU65_RS04210 (OPU65_04210) - 827014..827394 (-) 381 WP_265626961.1 hypothetical protein -
  OPU65_RS04215 (OPU65_04215) - 827391..827774 (-) 384 WP_265626962.1 HK97-gp10 family putative phage morphogenesis protein -
  OPU65_RS04220 (OPU65_04220) - 827767..828141 (-) 375 WP_265626963.1 phage head closure protein -
  OPU65_RS04225 (OPU65_04225) - 828071..828421 (-) 351 WP_265626964.1 head-tail connector protein -
  OPU65_RS04230 (OPU65_04230) - 828437..828886 (-) 450 Protein_843 collagen-like protein -
  OPU65_RS04235 (OPU65_04235) - 828908..830188 (-) 1281 WP_265626965.1 phage major capsid protein -
  OPU65_RS04240 (OPU65_04240) - 830225..830917 (-) 693 WP_265626966.1 head maturation protease, ClpP-related -
  OPU65_RS04245 (OPU65_04245) - 830901..832031 (-) 1131 WP_265626967.1 phage portal protein -
  OPU65_RS04250 (OPU65_04250) - 832212..832406 (-) 195 WP_409365388.1 DUF1056 family protein -
  OPU65_RS04255 (OPU65_04255) - 832416..834122 (-) 1707 WP_265626968.1 terminase large subunit -
  OPU65_RS04260 (OPU65_04260) - 834119..834634 (-) 516 WP_265626969.1 phage terminase small subunit P27 family -
  OPU65_RS04265 (OPU65_04265) - 834843..835178 (-) 336 WP_265626970.1 transglycosylase -
  OPU65_RS04270 (OPU65_04270) - 835138..835512 (-) 375 WP_265626971.1 HNH endonuclease signature motif containing protein -
  OPU65_RS04275 (OPU65_04275) - 835714..836373 (-) 660 WP_217620796.1 hypothetical protein -
  OPU65_RS04280 (OPU65_04280) - 836395..837342 (-) 948 WP_217620797.1 hypothetical protein -
  OPU65_RS04285 (OPU65_04285) - 837629..837886 (-) 258 WP_217620798.1 hypothetical protein -
  OPU65_RS04290 (OPU65_04290) - 838299..838679 (-) 381 WP_145685266.1 ArpU family phage packaging/lysis transcriptional regulator -
  OPU65_RS04295 (OPU65_04295) - 838792..839157 (-) 366 WP_186440857.1 YopX family protein -
  OPU65_RS04300 (OPU65_04300) - 839172..839687 (-) 516 WP_265626972.1 putative metallopeptidase -
  OPU65_RS04305 (OPU65_04305) - 839690..839860 (-) 171 WP_003185375.1 Fur-regulated basic protein FbpA -
  OPU65_RS04310 (OPU65_04310) - 839857..840396 (-) 540 WP_061578362.1 ERCC4 domain-containing protein -
  OPU65_RS04315 (OPU65_04315) - 840393..840830 (-) 438 WP_265626973.1 hypothetical protein -
  OPU65_RS04320 (OPU65_04320) - 840808..841179 (-) 372 WP_265626974.1 hypothetical protein -
  OPU65_RS04325 (OPU65_04325) - 841401..843833 (-) 2433 WP_265626975.1 phage/plasmid primase, P4 family -
  OPU65_RS04330 (OPU65_04330) - 843894..844334 (-) 441 WP_265626976.1 DUF669 domain-containing protein -
  OPU65_RS04335 (OPU65_04335) - 844334..845266 (-) 933 WP_265626977.1 AAA family ATPase -
  OPU65_RS04340 (OPU65_04340) - 845270..845830 (-) 561 WP_265626978.1 host-nuclease inhibitor Gam family protein -
  OPU65_RS04345 (OPU65_04345) - 846027..846293 (-) 267 WP_077736770.1 YqaH family protein -
  OPU65_RS04350 (OPU65_04350) - 846290..846853 (-) 564 WP_265626979.1 hypothetical protein -
  OPU65_RS04355 (OPU65_04355) - 846906..847178 (+) 273 WP_265626980.1 hypothetical protein -
  OPU65_RS04360 (OPU65_04360) - 847165..847329 (-) 165 WP_017474700.1 hypothetical protein -
  OPU65_RS04365 (OPU65_04365) - 847481..847792 (-) 312 WP_265626981.1 DNA-binding protein -
  OPU65_RS04370 (OPU65_04370) - 847807..848571 (-) 765 WP_265626982.1 Rha family transcriptional regulator -
  OPU65_RS04375 (OPU65_04375) - 848686..848904 (-) 219 WP_048355960.1 helix-turn-helix transcriptional regulator -
  OPU65_RS04380 (OPU65_04380) - 849034..849426 (+) 393 WP_265626983.1 helix-turn-helix domain-containing protein -
  OPU65_RS04385 (OPU65_04385) - 849407..850531 (+) 1125 WP_265626984.1 ImmA/IrrE family metallo-endopeptidase -
  OPU65_RS04390 (OPU65_04390) - 850572..851723 (+) 1152 WP_265626985.1 site-specific integrase -

Sequence


Protein


Download         Length: 158 a.a.        Molecular weight: 18276.16 Da        Isoelectric Point: 10.3521

>NTDB_id=756343 OPU65_RS04080 WP_059231482.1 802653..803129(-) (rok) [Bacillus paralicheniformis strain CamBx3]
MFNEREALRLRLEQLGDAEIQVMRELRKERQDIYSKLRELDRESDPAINKKSSLIDLASAAAEELKQSQHAADKHPPIKT
PSHTIFQSKTTIQREAALNILSRYEEGLKGIKLKTEIEKETGFPIKNMTTFMKSLMKHRPDIKKPARGHYILQKPKSL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=756343 OPU65_RS04080 WP_059231482.1 802653..803129(-) (rok) [Bacillus paralicheniformis strain CamBx3]
ATGTTCAACGAAAGAGAAGCGTTGCGTTTGAGACTTGAACAGCTGGGAGATGCGGAGATTCAGGTGATGCGGGAATTGCG
AAAAGAACGACAGGACATTTATTCGAAGCTGCGTGAATTGGATCGGGAGAGTGATCCCGCAATTAACAAAAAGTCTTCAT
TGATCGACTTGGCAAGCGCTGCAGCAGAGGAATTAAAACAATCCCAACATGCCGCTGACAAACATCCGCCCATCAAGACT
CCATCTCATACTATTTTCCAATCAAAAACAACCATACAGCGTGAGGCTGCCTTAAACATTTTAAGCCGCTATGAAGAGGG
CTTGAAAGGCATTAAGCTCAAAACTGAAATCGAGAAAGAAACCGGCTTTCCGATTAAAAACATGACGACATTTATGAAGA
GCTTGATGAAACATCGCCCAGATATTAAAAAGCCTGCCCGCGGCCATTATATTTTGCAAAAACCCAAAAGCCTTTAG

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rok Bacillus subtilis subsp. subtilis str. 168

44.444

100

0.506