Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OPU65_RS00695 Genome accession   NZ_CP110812
Coordinates   118644..119708 (+) Length   354 a.a.
NCBI ID   WP_265626327.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain CamBx3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113644..124708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OPU65_RS00655 (OPU65_00655) - 114069..115178 (+) 1110 WP_265626323.1 hypothetical protein -
  OPU65_RS00660 (OPU65_00660) - 115194..115508 (+) 315 WP_075752339.1 MTH1187 family thiamine-binding protein -
  OPU65_RS00665 (OPU65_00665) - 115569..115742 (-) 174 WP_003183486.1 DUF2759 domain-containing protein -
  OPU65_RS00670 (OPU65_00670) - 115898..116536 (+) 639 WP_265626325.1 MBL fold metallo-hydrolase -
  OPU65_RS00675 (OPU65_00675) - 116572..116817 (-) 246 WP_020452180.1 DUF2626 domain-containing protein -
  OPU65_RS00680 (OPU65_00680) - 117028..117405 (+) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  OPU65_RS00690 (OPU65_00690) - 117647..118486 (+) 840 WP_265626326.1 STAS domain-containing protein -
  OPU65_RS00695 (OPU65_00695) comGA 118644..119708 (+) 1065 WP_265626327.1 competence type IV pilus ATPase ComGA Machinery gene
  OPU65_RS00700 (OPU65_00700) comGB 119698..120732 (+) 1035 WP_265626330.1 competence type IV pilus assembly protein ComGB Machinery gene
  OPU65_RS00705 (OPU65_00705) comGC 120746..121039 (+) 294 WP_165427561.1 competence type IV pilus major pilin ComGC Machinery gene
  OPU65_RS00710 (OPU65_00710) comGD 121069..121482 (+) 414 WP_265626332.1 competence type IV pilus minor pilin ComGD -
  OPU65_RS00715 (OPU65_00715) comGE 121466..121813 (+) 348 WP_243943148.1 competence type IV pilus minor pilin ComGE -
  OPU65_RS00720 (OPU65_00720) comGF 121746..122210 (+) 465 WP_265626335.1 competence type IV pilus minor pilin ComGF -
  OPU65_RS00725 (OPU65_00725) comGG 122222..122587 (+) 366 WP_265626336.1 competence type IV pilus minor pilin ComGG -
  OPU65_RS00730 (OPU65_00730) - 122676..122858 (+) 183 WP_020452171.1 YqzE family protein -
  OPU65_RS00735 (OPU65_00735) - 122887..123207 (-) 321 WP_165427557.1 YqzG/YhdC family protein -
  OPU65_RS00740 (OPU65_00740) tapA 123485..124213 (+) 729 WP_265626339.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39751.29 Da        Isoelectric Point: 9.1063

>NTDB_id=756334 OPU65_RS00695 WP_265626327.1 118644..119708(+) (comGA) [Bacillus paralicheniformis strain CamBx3]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATVKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLELGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GDMKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=756334 OPU65_RS00695 WP_265626327.1 118644..119708(+) (comGA) [Bacillus paralicheniformis strain CamBx3]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTGTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTCTCTTCGATGGACATTGGTGAAAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGACT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTTTCATTGTTTCCGAGTGCAACAGTTAAGCTGCTGTCTTTTCTAA
AGCATTCCCACGGATTAATGCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACTCTCTATTCTTTAATTGAATAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTCGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTTAATGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTTG
GAGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCAAAAGGCGCGATATACAGACTGCTCGAGCTCGGCGTTGATATGACAGAAATTGAACAAACATTGGTCGC
AATCAGCGCCCAACGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATGAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.514

100

0.675