Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QJP60_RS10380 Genome accession   NZ_CP124415
Coordinates   2145289..2146722 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain AVS0223     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2140289..2151722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJP60_RS10350 (QJP60_10350) yrbG 2140847..2141824 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  QJP60_RS10355 (QJP60_10355) kdsD 2141838..2142824 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QJP60_RS10360 (QJP60_10360) kdsC 2142845..2143411 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QJP60_RS10365 (QJP60_10365) lptC 2143408..2143983 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QJP60_RS10370 (QJP60_10370) lptA 2143952..2144509 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QJP60_RS10375 (QJP60_10375) lptB 2144516..2145241 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QJP60_RS10380 (QJP60_10380) rpoN/rpoN1 2145289..2146722 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  QJP60_RS10385 (QJP60_10385) hpf 2146745..2147032 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QJP60_RS10390 (QJP60_10390) ptsN 2147150..2147641 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QJP60_RS10395 (QJP60_10395) rapZ 2147687..2148541 (+) 855 WP_000243741.1 RNase adapter RapZ -
  QJP60_RS10400 (QJP60_10400) npr 2148538..2148810 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QJP60_RS10405 (QJP60_10405) yrbL 2149023..2149655 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  QJP60_RS10410 (QJP60_10410) mtgA 2149652..2150380 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QJP60_RS10415 (QJP60_10415) elbB 2150377..2151030 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=756233 QJP60_RS10380 WP_000809051.1 2145289..2146722(+) (rpoN/rpoN1) [Escherichia coli strain AVS0223]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=756233 QJP60_RS10380 WP_000809051.1 2145289..2146722(+) (rpoN/rpoN1) [Escherichia coli strain AVS0223]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAAGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGATGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
AGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGCCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465