Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ONZ66_RS01760 Genome accession   NZ_CP110647
Coordinates   357346..358785 (+) Length   479 a.a.
NCBI ID   WP_111588921.1    Uniprot ID   A0AAX1PHB2
Organism   Aeromonas salmonicida strain ZAS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352346..363785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ66_RS01730 (ONZ66_01730) mlaF 352923..353723 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  ONZ66_RS01735 (ONZ66_01735) - 353931..354926 (+) 996 WP_034524103.1 KpsF/GutQ family sugar-phosphate isomerase -
  ONZ66_RS01740 (ONZ66_01740) kdsC 354926..355480 (+) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ONZ66_RS01745 (ONZ66_01745) lptC 355477..356037 (+) 561 WP_021140208.1 LPS export ABC transporter periplasmic protein LptC -
  ONZ66_RS01750 (ONZ66_01750) lptA 356024..356551 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  ONZ66_RS01755 (ONZ66_01755) lptB 356555..357280 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  ONZ66_RS01760 (ONZ66_01760) rpoN/rpoN1 357346..358785 (+) 1440 WP_111588921.1 RNA polymerase factor sigma-54 Machinery gene
  ONZ66_RS01765 (ONZ66_01765) hpf 358807..359094 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ONZ66_RS01770 (ONZ66_01770) ptsN 359097..359543 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ONZ66_RS01775 (ONZ66_01775) rapZ 359586..360452 (+) 867 WP_271774080.1 RNase adapter RapZ -
  ONZ66_RS01780 (ONZ66_01780) npr 360473..360745 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  ONZ66_RS01785 (ONZ66_01785) - 360764..362668 (-) 1905 WP_271774081.1 BglG family transcription antiterminator -
  ONZ66_RS01790 (ONZ66_01790) dagF 362772..363512 (-) 741 WP_271774082.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53891.84 Da        Isoelectric Point: 4.3459

>NTDB_id=755640 ONZ66_RS01760 WP_111588921.1 357346..358785(+) (rpoN/rpoN1) [Aeromonas salmonicida strain ZAS]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQVAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=755640 ONZ66_RS01760 WP_111588921.1 357346..358785(+) (rpoN/rpoN1) [Aeromonas salmonicida strain ZAS]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACTTGGGAAGATGTCTATTCAGCTGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCTGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGTGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCCGTGCTCAAACGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTCGAACTGAACCCGG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484