Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OOZ27_RS14385 Genome accession   NZ_CP110634
Coordinates   2691467..2691877 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain MG-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2686467..2696877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOZ27_RS14355 (OOZ27_14355) - 2686553..2686693 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  OOZ27_RS14360 (OOZ27_14360) yqeF 2686999..2687730 (-) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  OOZ27_RS14365 (OOZ27_14365) cwlH 2687983..2688735 (-) 753 WP_015714296.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  OOZ27_RS14370 (OOZ27_14370) yqeD 2688922..2689548 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  OOZ27_RS14375 (OOZ27_14375) gnd 2689567..2690460 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  OOZ27_RS14380 (OOZ27_14380) yqeB 2690712..2691434 (+) 723 WP_010886572.1 hypothetical protein -
  OOZ27_RS14385 (OOZ27_14385) nucA/comI 2691467..2691877 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  OOZ27_RS14390 (OOZ27_14390) - 2692073..2692423 (+) 351 Protein_2788 sigma-70 family RNA polymerase sigma factor -
  OOZ27_RS14395 (OOZ27_14395) spoIVCA 2692451..2693911 (-) 1461 WP_243737903.1 site-specific DNA recombinase SpoIVCA -
  OOZ27_RS14400 (OOZ27_14400) - 2693869..2694047 (-) 179 Protein_2790 hypothetical protein -
  OOZ27_RS14405 (OOZ27_14405) arsC 2694402..2694821 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  OOZ27_RS14410 (OOZ27_14410) acr3 2694833..2695873 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  OOZ27_RS14415 (OOZ27_14415) arsK 2695896..2696336 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  OOZ27_RS14420 (OOZ27_14420) arsR 2696397..2696714 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=755515 OOZ27_RS14385 WP_009967785.1 2691467..2691877(-) (nucA/comI) [Bacillus subtilis strain MG-1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=755515 OOZ27_RS14385 WP_009967785.1 2691467..2691877(-) (nucA/comI) [Bacillus subtilis strain MG-1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529