Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAbrini_RS16530 Genome accession   NZ_CP110420
Coordinates   3563148..3564203 (+) Length   351 a.a.
NCBI ID   WP_013237892.1    Uniprot ID   A0A162KYQ6
Organism   Clostridium autoethanogenum strain S232868     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3558148..3569203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAbrini_RS16510 (LAbrini_16520) - 3558465..3559112 (+) 648 WP_013237888.1 O-methyltransferase -
  LAbrini_RS16515 (LAbrini_16525) - 3559114..3560340 (+) 1227 WP_013237889.1 U32 family peptidase -
  LAbrini_RS16520 (LAbrini_16530) - 3560426..3562090 (+) 1665 WP_013237890.1 penicillin-binding transpeptidase domain-containing protein -
  LAbrini_RS16525 (LAbrini_16535) sigK 3562173..3562874 (+) 702 WP_013237891.1 RNA polymerase sporulation sigma factor SigK -
  LAbrini_RS16530 (LAbrini_16540) pilT 3563148..3564203 (+) 1056 WP_013237892.1 type IV pilus twitching motility protein PilT Machinery gene
  LAbrini_RS16535 (LAbrini_16545) - 3564339..3565613 (+) 1275 WP_013237893.1 glycosyltransferase family 2 protein -
  LAbrini_RS16540 (LAbrini_16550) ftsA 3566000..3567271 (+) 1272 WP_029170054.1 cell division protein FtsA -
  LAbrini_RS16545 (LAbrini_16555) ftsZ 3567290..3568399 (+) 1110 WP_013237895.1 cell division protein FtsZ -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38821.29 Da        Isoelectric Point: 8.5799

>NTDB_id=754968 LAbrini_RS16530 WP_013237892.1 3563148..3564203(+) (pilT) [Clostridium autoethanogenum strain S232868]
MLSLKDLLEITIKEEASDLHLTVGIPPVIRVNGELEVLGKEKLQPSDTERFSKEILDKDYDKYMKIGEIDISFSVAGLGR
FRVNIFKQRGSNAIAVRVVGLKIPTLNELKFPQVIKNLLSYKKGLILVTGPTGCGKSTTLAAMINEINCTRAAHIITLED
PIEYLHKHNKSIINQREIGKDSLSYANALKSVLREDPDVILVGEMRDLETISVVLTASETGHLVFSTLHTIGAAKTIDRI
VDVFPPYQQQQIKIQLAAVLRGIVSQQLIHREDGKGRVAAVETMIATPAIKNMIREGKTYQIESSMQTGSKYGMKTMDIA
LAELYKKGLISYESAIAYACDANIITKIISL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=754968 LAbrini_RS16530 WP_013237892.1 3563148..3564203(+) (pilT) [Clostridium autoethanogenum strain S232868]
TTGCTATCATTAAAAGATTTATTAGAAATAACTATTAAAGAAGAGGCATCAGATTTGCACCTTACTGTAGGTATACCTCC
TGTAATAAGGGTAAATGGAGAACTTGAAGTACTTGGAAAAGAAAAACTGCAGCCATCAGATACAGAAAGATTTAGTAAAG
AAATATTAGATAAAGATTATGATAAATATATGAAAATTGGAGAAATCGACATATCATTTTCTGTAGCTGGACTTGGCAGA
TTTAGAGTAAATATTTTCAAGCAAAGGGGAAGTAATGCCATTGCAGTGAGAGTTGTAGGATTGAAAATACCAACTTTAAA
TGAACTTAAATTTCCACAGGTAATAAAAAATCTTTTATCTTATAAAAAAGGACTGATTTTGGTAACAGGTCCTACAGGTT
GTGGAAAAAGTACGACTTTAGCAGCTATGATAAATGAAATAAATTGTACAAGAGCTGCACATATAATAACTTTAGAAGAT
CCTATAGAATATTTACATAAGCACAACAAGTCTATAATAAATCAGAGGGAAATAGGGAAAGATTCCTTAAGCTATGCTAA
TGCTTTAAAGTCAGTACTTAGAGAAGATCCAGATGTAATTTTAGTGGGAGAAATGAGAGACCTTGAAACTATATCTGTTG
TGCTTACGGCTTCTGAAACCGGACATCTTGTATTTTCGACGTTACATACTATAGGAGCAGCTAAAACTATAGATAGAATT
GTAGATGTATTTCCACCGTATCAACAACAGCAGATAAAAATACAATTAGCAGCAGTTTTAAGGGGAATAGTATCTCAGCA
GCTTATACATAGAGAAGATGGTAAGGGAAGAGTAGCAGCAGTTGAGACTATGATTGCTACTCCAGCCATTAAAAATATGA
TAAGAGAAGGAAAAACCTATCAAATAGAATCTTCAATGCAAACCGGGTCAAAGTATGGTATGAAGACAATGGATATAGCA
CTGGCGGAACTTTATAAAAAAGGATTAATATCTTATGAGTCTGCTATTGCCTATGCTTGTGATGCAAATATAATAACTAA
AATAATTTCATTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A162KYQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.294

96.866

0.487
  pilT Vibrio cholerae strain A1552

50.599

95.157

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.599

95.157

0.481
  pilT Pseudomonas aeruginosa PAK

49.558

96.581

0.479
  pilT Neisseria meningitidis 8013

50

95.726

0.479
  pilT Neisseria gonorrhoeae MS11

49.552

95.442

0.473
  pilT Pseudomonas stutzeri DSM 10701

48.673

96.581

0.47
  pilT Acinetobacter baumannii strain A118

49.107

95.726

0.47
  pilT Acinetobacter nosocomialis M2

49.107

95.726

0.47
  pilT Acinetobacter baumannii D1279779

49.107

95.726

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.839

99.145

0.464
  pilT Legionella pneumophila strain Lp02

46.667

98.291

0.459
  pilT Legionella pneumophila strain ERS1305867

46.667

98.291

0.459
  pilU Acinetobacter baylyi ADP1

40.407

98.006

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.601

100

0.396
  pilU Vibrio cholerae strain A1552

39.942

97.721

0.39