Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LFL96_RS02115 Genome accession   NZ_CP110395
Coordinates   479709..481226 (-) Length   505 a.a.
NCBI ID   WP_280997516.1    Uniprot ID   -
Organism   Paraburkholderia sp. D15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474709..486226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFL96_RS02090 (LFL96_02090) - 475575..476210 (-) 636 WP_280997511.1 LON peptidase substrate-binding domain-containing protein -
  LFL96_RS02095 (LFL96_02095) rapZ 476250..477143 (-) 894 WP_280997513.1 RNase adapter RapZ -
  LFL96_RS02100 (LFL96_02100) hprK 477261..478229 (-) 969 WP_064266564.1 HPr(Ser) kinase/phosphatase -
  LFL96_RS02105 (LFL96_02105) - 478369..478884 (-) 516 WP_281000874.1 PTS sugar transporter subunit IIA -
  LFL96_RS02110 (LFL96_02110) raiA 479183..479539 (-) 357 WP_280997515.1 ribosome-associated translation inhibitor RaiA -
  LFL96_RS02115 (LFL96_02115) rpoN/rpoN1 479709..481226 (-) 1518 WP_280997516.1 RNA polymerase factor sigma-54 Machinery gene
  LFL96_RS02120 (LFL96_02120) lptB 481512..482297 (-) 786 WP_280997517.1 LPS export ABC transporter ATP-binding protein -
  LFL96_RS02125 (LFL96_02125) lptA 482294..482965 (-) 672 WP_280997519.1 lipopolysaccharide transport periplasmic protein LptA -
  LFL96_RS02130 (LFL96_02130) lptC 482987..483598 (-) 612 WP_280997521.1 LPS export ABC transporter periplasmic protein LptC -
  LFL96_RS02135 (LFL96_02135) - 483595..484155 (-) 561 WP_280997522.1 HAD family hydrolase -
  LFL96_RS02140 (LFL96_02140) kdsD 484155..485138 (-) 984 WP_280997524.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55541.56 Da        Isoelectric Point: 5.0653

>NTDB_id=754864 LFL96_RS02115 WP_280997516.1 479709..481226(-) (rpoN/rpoN1) [Paraburkholderia sp. D15]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVASDGSLIAQAPNSSAPPDQM
GGNTSSSSTSSNDRAENGEPQGVDEYNGLSGDSNGDTSQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALITFLIESLDDDGYLAATLDEVLADLPEELEVDVDELNAALALLHSFDPAGVGARSASECLKLQLLRLDTSP
TRTLALDIVAHHLELLAARDFTRLRKHLKANDDDLRDAHLLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSYFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=754864 LFL96_RS02115 WP_280997516.1 479709..481226(-) (rpoN/rpoN1) [Paraburkholderia sp. D15]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACGCCACAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTCGCCATGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTGCGGGTGGCGTCCGACGGCTCGCTGATCGCGCAGGCGCCGAATTCGTCCGCGCCGCCCGATCAGATG
GGCGGCAACACGTCGTCCTCGTCGACGAGCAGCAACGACCGCGCGGAGAACGGCGAACCGCAAGGCGTGGACGAATACAA
CGGCCTCTCCGGCGACAGCAACGGTGACACGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCATCGGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCCAGCACCTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTGACC
CAGGCGGGCCAACGCGACCGGGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGACGGCTACCTCGCCGCCACGCT
CGATGAAGTGCTGGCCGATCTGCCTGAAGAACTCGAAGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTGCTGCATA
GCTTCGATCCGGCGGGCGTCGGCGCGCGCTCCGCGTCGGAATGTCTGAAGCTGCAATTGCTGCGGCTCGACACCTCGCCC
ACGCGCACGCTTGCGCTCGACATCGTGGCCCATCATCTGGAATTGCTCGCGGCGCGCGACTTCACGCGTCTGCGCAAACA
CCTGAAGGCCAACGACGACGATCTGCGCGACGCACACCTGCTGATCCGCTCGCTCGAACCGTTCCCCGGCGCGGCGTACG
GCAAGGCCGAAGCCGACTACGTGGTGCCCGACATCATGGTGCGCAAAACGGCACAAGGGTGGCAGGCCGAACTGAATCCC
GAAGTCGTGCCGAAACTGCGGATTAACCACCTGTACGCGAATATTCTGCGCAATAACCGGGGCGATCCGGGCAGTGGTTC
GTTGCGTCAGCAACTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAACGTTTCGAAACGATCCTGCGGGTCGCGC
AAGCCATTGTGGAGCGTCAAAAGAGCTATTTCGTACATGGCGAAATTGCCATGCGCCCCTTGGTTTTGAGGGAGATTGCT
GATACGCTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTTTCGACGGACACGGGCGGCGCGGCATCCTCCACAGCGATCCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTCGCGAAGTATCGCGAAGCACTGAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.733

100

0.667