Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PALA52_RS22930 Genome accession   NZ_CP110347
Coordinates   4964581..4966074 (+) Length   497 a.a.
NCBI ID   WP_024914711.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4959581..4971074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA52_RS22900 (PALA52_04547) - 4959793..4960602 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  PALA52_RS22905 (PALA52_04548) kdsD 4961016..4961996 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  PALA52_RS22910 (PALA52_04549) - 4961996..4962535 (+) 540 WP_058141249.1 KdsC family phosphatase -
  PALA52_RS22915 (PALA52_04550) lptC 4962544..4963116 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PALA52_RS22920 (PALA52_04551) lptA 4963103..4963630 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PALA52_RS22925 (PALA52_04552) lptB 4963630..4964355 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PALA52_RS22930 (PALA52_04553) rpoN/rpoN1 4964581..4966074 (+) 1494 WP_024914711.1 RNA polymerase factor sigma-54 Machinery gene
  PALA52_RS22935 (PALA52_04554) hpf 4966152..4966460 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PALA52_RS22940 (PALA52_04555) ptsN 4966474..4966938 (+) 465 WP_024914710.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PALA52_RS22945 (PALA52_04556) rapZ 4966940..4967800 (+) 861 WP_024914709.1 RNase adapter RapZ -
  PALA52_RS22950 (PALA52_04557) - 4967830..4968102 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PALA52_RS22955 (PALA52_04558) - 4968199..4969131 (-) 933 WP_263957276.1 ZIP family metal transporter -
  PALA52_RS22960 (PALA52_04559) - 4969150..4969761 (-) 612 WP_024914706.1 superoxide dismutase -
  PALA52_RS22965 (PALA52_04560) - 4969774..4970223 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56041.22 Da        Isoelectric Point: 4.4351

>NTDB_id=754271 PALA52_RS22930 WP_024914711.1 4964581..4966074(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA52]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSSDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELEVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=754271 PALA52_RS22930 WP_024914711.1 4964581..4966074(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA52]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCGCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCGCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATTCCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAGCGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGCATGATCGCA
GTGACCATCATCGATAGCATCAACAACGACGGCTACCTCGAAGAGTCCCTCGAAGAGATCCTCGCCGCCATCGATCCGGA
ACTGGAGGTCGAACTCGACGAAGTGGAAGTCGTGCTGCGTCGCATCCAGCAGCTGGAGCCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCATCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTTGGCGGCCGCGACTACAGCCAGTTGATGCGACGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAACTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAT
GCGACCTACGCCGGCATGGTCCGTCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAAAGCCGCAACGAAACGCTGATGAAGGTCGCGACCCAAATCGTCGAGCACCAGCGTGGTT
TCCTCGACTACGGTGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAGGCGGTGGGCATGCACGAGTCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCACACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGCACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

47.047

100

0.481