Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OM953_RS16445 Genome accession   NZ_CP110301
Coordinates   3754363..3756099 (-) Length   578 a.a.
NCBI ID   WP_007965359.1    Uniprot ID   A0A7Z7J2H4
Organism   Xanthomonas citri pv. fuscans strain CFBP 6533     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3749363..3761099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM953_RS16435 (OM953_16435) - 3750575..3752188 (+) 1614 WP_007965357.1 HAMP domain-containing sensor histidine kinase -
  OM953_RS16440 (OM953_16440) - 3752458..3753913 (+) 1456 Protein_3209 sigma-54 dependent transcriptional regulator -
  OM953_RS16445 (OM953_16445) pilB 3754363..3756099 (-) 1737 WP_007965359.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM953_RS16450 (OM953_16450) pilA2 3756141..3756554 (-) 414 WP_089111877.1 pilin Machinery gene
  OM953_RS16460 (OM953_16460) comP 3756652..3757080 (-) 429 WP_007962467.1 pilin Machinery gene
  OM953_RS16465 (OM953_16465) pilC 3757426..3758685 (+) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  OM953_RS16470 (OM953_16470) - 3758692..3759555 (+) 864 WP_003491180.1 A24 family peptidase -
  OM953_RS16475 (OM953_16475) coaE 3759569..3760180 (+) 612 WP_099802201.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62656.77 Da        Isoelectric Point: 5.7586

>NTDB_id=753987 OM953_RS16445 WP_007965359.1 3754363..3756099(-) (pilB) [Xanthomonas citri pv. fuscans strain CFBP 6533]
MNVAIGSNLVGITGIARRLVQDGALDEVSARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=753987 OM953_RS16445 WP_007965359.1 3754363..3756099(-) (pilB) [Xanthomonas citri pv. fuscans strain CFBP 6533]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGTCTTGTTCAGGATGGTGCGCTTGATGA
GGTGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTGCCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAGCTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCATTCGACGCCAAC
CAGAACGCAGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGTTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTTCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAATGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAGGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCATTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z7J2H4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilB Vibrio cholerae strain A1552

48.789

100

0.488
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445