Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | OMK57_RS13245 | Genome accession | NZ_CP110264 |
| Coordinates | 2647156..2647581 (-) | Length | 141 a.a. |
| NCBI ID | WP_044154477.1 | Uniprot ID | - |
| Organism | Bacillus halotolerans strain HMB20199 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2640135..2664630 | 2647156..2647581 | within | 0 |
Gene organization within MGE regions
Location: 2640135..2664630
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OMK57_RS13200 (OMK57_13200) | yqeH | 2640135..2641235 (-) | 1101 | WP_059335448.1 | ribosome biogenesis GTPase YqeH | - |
| OMK57_RS13205 (OMK57_13205) | - | 2641238..2641756 (-) | 519 | WP_003226126.1 | YqeG family HAD IIIA-type phosphatase | - |
| OMK57_RS13210 (OMK57_13210) | - | 2642118..2642258 (+) | 141 | WP_003226124.1 | sporulation histidine kinase inhibitor Sda | - |
| OMK57_RS13215 (OMK57_13215) | - | 2642396..2642530 (-) | 135 | WP_010335019.1 | hypothetical protein | - |
| OMK57_RS13220 (OMK57_13220) | - | 2642608..2643339 (-) | 732 | WP_044154481.1 | SGNH/GDSL hydrolase family protein | - |
| OMK57_RS13225 (OMK57_13225) | - | 2643586..2644338 (-) | 753 | WP_069487172.1 | N-acetylmuramoyl-L-alanine amidase | - |
| OMK57_RS13230 (OMK57_13230) | - | 2644533..2645159 (+) | 627 | WP_059335451.1 | TVP38/TMEM64 family protein | - |
| OMK57_RS13235 (OMK57_13235) | gnd | 2645180..2646073 (-) | 894 | WP_059335452.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| OMK57_RS13240 (OMK57_13240) | - | 2646394..2647122 (+) | 729 | WP_044154478.1 | hypothetical protein | - |
| OMK57_RS13245 (OMK57_13245) | nucA/comI | 2647156..2647581 (-) | 426 | WP_044154477.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| OMK57_RS13250 (OMK57_13250) | sigK | 2647758..2648486 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| OMK57_RS13255 (OMK57_13255) | - | 2648672..2648770 (-) | 99 | Protein_2570 | recombinase | - |
| OMK57_RS13260 (OMK57_13260) | - | 2649126..2650940 (-) | 1815 | WP_069487171.1 | AAA family ATPase | - |
| OMK57_RS13265 (OMK57_13265) | - | 2651407..2655027 (-) | 3621 | WP_264797978.1 | DEAD/DEAH box helicase | - |
| OMK57_RS13270 (OMK57_13270) | - | 2654954..2655865 (-) | 912 | WP_069487169.1 | hypothetical protein | - |
| OMK57_RS13275 (OMK57_13275) | - | 2656353..2657480 (-) | 1128 | WP_069487168.1 | Rap family tetratricopeptide repeat protein | - |
| OMK57_RS13280 (OMK57_13280) | - | 2657890..2658024 (+) | 135 | WP_069487167.1 | hypothetical protein | - |
| OMK57_RS13285 (OMK57_13285) | - | 2658159..2659010 (-) | 852 | WP_069487166.1 | aldo/keto reductase | - |
| OMK57_RS13290 (OMK57_13290) | - | 2659181..2659624 (+) | 444 | WP_029318107.1 | MerR family transcriptional regulator | - |
| OMK57_RS13295 (OMK57_13295) | - | 2659825..2659974 (-) | 150 | WP_237730345.1 | helix-turn-helix domain-containing protein | - |
| OMK57_RS13300 (OMK57_13300) | - | 2660557..2662482 (+) | 1926 | WP_069487165.1 | ribonuclease YeeF family protein | - |
| OMK57_RS13305 (OMK57_13305) | - | 2662496..2662783 (+) | 288 | WP_014664698.1 | hypothetical protein | - |
| OMK57_RS13310 (OMK57_13310) | - | 2662820..2663638 (-) | 819 | WP_069487164.1 | N-acetylmuramoyl-L-alanine amidase | - |
| OMK57_RS13315 (OMK57_13315) | - | 2663683..2664105 (-) | 423 | WP_069487163.1 | holin family protein | - |
Sequence
Protein
Download Length: 141 a.a. Molecular weight: 15556.74 Da Isoelectric Point: 5.2215
>NTDB_id=753579 OMK57_RS13245 WP_044154477.1 2647156..2647581(-) (nucA/comI) [Bacillus halotolerans strain HMB20199]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ
Nucleotide
Download Length: 426 bp
>NTDB_id=753579 OMK57_RS13245 WP_044154477.1 2647156..2647581(-) (nucA/comI) [Bacillus halotolerans strain HMB20199]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
61.29 |
87.943 |
0.539 |