Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QEP20_RS12945 Genome accession   NZ_CP123977
Coordinates   2507927..2508337 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain WH60A     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2502927..2513337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP20_RS12915 (QEP20_12915) - 2503039..2503179 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  QEP20_RS12920 (QEP20_12920) yqeF 2503485..2504216 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  QEP20_RS12925 (QEP20_12925) cwlH 2504468..2505220 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QEP20_RS12930 (QEP20_12930) yqeD 2505407..2506033 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  QEP20_RS12935 (QEP20_12935) gnd 2506052..2506920 (-) 869 Protein_2498 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QEP20_RS12940 (QEP20_12940) yqeB 2507172..2507894 (+) 723 WP_010886572.1 hypothetical protein -
  QEP20_RS12945 (QEP20_12945) nucA/comI 2507927..2508337 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QEP20_RS12950 (QEP20_12950) - 2508533..2508880 (+) 348 Protein_2501 sigma-70 family RNA polymerase sigma factor -
  QEP20_RS12955 (QEP20_12955) spoIVCA 2508911..2510371 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  QEP20_RS12960 (QEP20_12960) - 2510329..2510507 (-) 179 Protein_2503 hypothetical protein -
  QEP20_RS12965 (QEP20_12965) arsC 2510862..2511281 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  QEP20_RS12970 (QEP20_12970) acr3 2511293..2512333 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  QEP20_RS12975 (QEP20_12975) yqcK 2512356..2512796 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  QEP20_RS12980 (QEP20_12980) arsR 2512855..2513172 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=753568 QEP20_RS12945 WP_009967785.1 2507927..2508337(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain WH60A]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=753568 QEP20_RS12945 WP_009967785.1 2507927..2508337(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain WH60A]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529