Detailed information NTG0753545

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: OMK57_RS05715
• Genome accession: NZ_CP110264
• Coordinates: 1169455..1173159 (+)
• Length: 1234 a.a.
• NCBI ID: WP_069486349.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain HMB20199
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1164455..1178159

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OMK57_RS05700 (OMK57_05700)	-	1164903..1165217 (-)	315	WP_044156484.1	four-helix bundle copper-binding protein	-
OMK57_RS05705 (OMK57_05705)	-	1165346..1165783 (-)	438	WP_044156482.1	ferritin family protein	-
OMK57_RS05710 (OMK57_05710)	addB	1165968..1169468 (+)	3501	WP_069486348.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
OMK57_RS05715 (OMK57_05715)	addA	1169455..1173159 (+)	3705	WP_069486349.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
OMK57_RS05720 (OMK57_05720)	sbcD	1173231..1174409 (+)	1179	WP_044156478.1	exonuclease subunit SbcD	-
OMK57_RS05725 (OMK57_05725)	sbcC	1174406..1177798 (+)	3393	WP_069486350.1	exonuclease subunit SbcC	-
OMK57_RS05730 (OMK57_05730)	-	1177812..1178114 (+)	303	WP_044156476.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141462.93 Da
• Isoelectric Point: 5.1008

>NTDB_id=753545 OMK57_RS05715 WP_069486349.1 1169455..1173159(+) (addA) [Bacillus halotolerans strain HMB20199]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLADMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENSETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKALERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGAKWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=753545 OMK57_RS05715 WP_069486349.1 1169455..1173159(+) (addA) [Bacillus halotolerans strain HMB20199]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGATTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCAGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTGAAACGCCCAGGATCGCTGCATATCCGACGCCAGCTGTCGCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTATCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGTTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCCCGTTCCCACCCTGATCCGGAGACATGGCTGAAAAGTTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTCCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTACGAAATGGCGCCAAAAAACTGCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGACATGGAGCCTGTCATTGAAACACTCGTACA
GCTTGTTATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATCGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTTGTCGATGAATACCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAATCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTCTCCAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGATTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAGCGAAACGGAAACAGAGCTGCTGTTGATTGACAAGTCAG
AGGAGGCGGACGCAGGCGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAGGAACTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAGCTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTCTATCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCTTTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ATCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGTTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTCCCAGTCGTTTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTGGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGCGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCTCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGGCGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGGGAA
AAACTGATCGGCGCTAAATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	93.436	100	0.934