Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OKW95_RS16765 Genome accession   NZ_CP110254
Coordinates   3560580..3561380 (-) Length   266 a.a.
NCBI ID   WP_323739746.1    Uniprot ID   -
Organism   Acinetobacter oleivorans strain S4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3555580..3566380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW95_RS16745 (OKW95_16750) - 3556646..3557344 (-) 699 WP_004794712.1 YfgM family protein -
  OKW95_RS16750 (OKW95_16755) hisS 3557372..3558664 (-) 1293 WP_013198977.1 histidine--tRNA ligase -
  OKW95_RS16755 (OKW95_16760) ispG 3558661..3559776 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OKW95_RS16760 (OKW95_16765) - 3559798..3560589 (-) 792 WP_005302244.1 helix-turn-helix domain-containing protein -
  OKW95_RS16765 (OKW95_16770) pilF 3560580..3561380 (-) 801 WP_323739746.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OKW95_RS16770 (OKW95_16775) rlmN 3561396..3562631 (-) 1236 WP_005302240.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OKW95_RS16775 (OKW95_16780) ndk 3562755..3563186 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  OKW95_RS16780 (OKW95_16785) iscX 3563300..3563500 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  OKW95_RS16785 (OKW95_16790) - 3563600..3564151 (-) 552 WP_005302237.1 phosphatase PAP2 family protein -
  OKW95_RS16790 (OKW95_16795) - 3564176..3565465 (-) 1290 WP_323740291.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30141.36 Da        Isoelectric Point: 8.7553

>NTDB_id=753390 OKW95_RS16765 WP_323739746.1 3560580..3561380(-) (pilF) [Acinetobacter oleivorans strain S4]
MSTPKLKTVLCMGVAVAFLVSGCQTTHVQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
TNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=753390 OKW95_RS16765 WP_323739746.1 3560580..3561380(-) (pilF) [Acinetobacter oleivorans strain S4]
TTGAGTACTCCAAAGTTAAAAACGGTATTATGTATGGGGGTTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TGTGCAAAAGAAAGACCCAGAAAAAGCGGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTACTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAACTATGGTACGTACTTGTATCAAATGGAACGCTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTAGGGCGTATTTATTTAAAATTGGGTGATGTT
ACGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCGAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733