Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OL235_RS09560 Genome accession   NZ_CP110237
Coordinates   2054358..2055746 (-) Length   462 a.a.
NCBI ID   WP_275469000.1    Uniprot ID   A0AAF0CUN7
Organism   Vagococcus intermedius strain STAA25     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2049358..2060746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OL235_RS09540 (OL235_09540) cysS 2049484..2050896 (-) 1413 WP_275468996.1 cysteine--tRNA ligase -
  OL235_RS09545 (OL235_09545) gltX 2051219..2052682 (-) 1464 WP_275468997.1 glutamate--tRNA ligase -
  OL235_RS09550 (OL235_09550) ispF 2052675..2053178 (-) 504 WP_275468998.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  OL235_RS09555 (OL235_09555) - 2053206..2054327 (-) 1122 WP_275468999.1 PIN/TRAM domain-containing protein -
  OL235_RS09560 (OL235_09560) radA 2054358..2055746 (-) 1389 WP_275469000.1 DNA repair protein RadA Machinery gene
  OL235_RS09565 (OL235_09565) - 2055918..2056448 (-) 531 WP_275469001.1 dUTP diphosphatase -
  OL235_RS09570 (OL235_09570) - 2056594..2056854 (-) 261 WP_275469002.1 metal-sensitive transcriptional regulator -
  OL235_RS09575 (OL235_09575) - 2056873..2058522 (-) 1650 WP_275469003.1 FAD-dependent oxidoreductase -
  OL235_RS09580 (OL235_09580) - 2058522..2058836 (-) 315 WP_275469004.1 rhodanese-like domain-containing protein -
  OL235_RS09585 (OL235_09585) - 2059024..2060067 (+) 1044 WP_275469005.1 M42 family metallopeptidase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50520.19 Da        Isoelectric Point: 7.3490

>NTDB_id=753333 OL235_RS09560 WP_275469000.1 2054358..2055746(-) (radA) [Vagococcus intermedius strain STAA25]
MAKKAKTQFECQACGYISPKYLGRCPNCGQWNEMLEVKLQEEVSRNSRVSLAGKRTTATKLGEVASKKEPRVKTKLGELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLHQSGGKVLYVSGEESAEQIKMRAERLSIEGNDFFVYPETDMSNIRQ
TIEELAPDYVIIDSIQTMMQPEISSATGSVSQVRETTAELMQIAKTNGIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMKEQGLVEVQNPSEAFLEERIDGATGSAIVVSMEGTRPILVEVQSLVTPTMFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYKEKGTKPTECFVGEIGLTG
EIRRVNRIEQRVVEAQKLGFKKMYLPKNNLQGWEAPKGIEIVGVATLGEALHKIFEKSEAAF

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=753333 OL235_RS09560 WP_275469000.1 2054358..2055746(-) (radA) [Vagococcus intermedius strain STAA25]
ATGGCGAAGAAAGCAAAAACACAGTTTGAATGCCAAGCTTGTGGGTATATCTCTCCGAAATATTTGGGACGTTGTCCAAA
TTGTGGGCAATGGAATGAAATGTTGGAAGTAAAACTGCAAGAGGAAGTATCACGTAATAGTCGTGTTAGTTTGGCAGGCA
AGCGAACAACAGCTACTAAATTAGGGGAAGTTGCATCTAAGAAAGAACCACGTGTGAAAACAAAATTAGGTGAACTGAAT
CGTGTGCTAGGTGGCGGTGTCGTACCAGGTTCACTAGTTTTAATTGGTGGAGATCCGGGGATTGGAAAGTCAACTTTATT
GTTACAAGTATCGCAACAATTACATCAAAGTGGTGGGAAAGTACTCTATGTTTCAGGTGAAGAAAGTGCTGAACAGATTA
AGATGCGAGCGGAACGTTTAAGCATTGAGGGTAACGATTTTTTTGTTTACCCTGAAACCGATATGTCTAATATCCGCCAA
ACTATTGAAGAGTTGGCTCCTGACTACGTTATCATTGATTCAATTCAAACGATGATGCAACCAGAAATTTCCAGTGCAAC
GGGCAGTGTTAGTCAAGTGCGTGAAACAACAGCTGAATTAATGCAGATTGCAAAAACAAATGGTATTGCTATTTTTATTG
TTGGCCATGTTACAAAGGAAGGGTCGTTGGCAGGACCGAGAATGTTAGAACACATGGTAGATACTGTGTTGTACTTTGAA
GGGGATCGCCATCACACATTTAGGATACTAAGAGCGGTCAAAAATCGTTTTGGTTCAACCAATGAGATTGGTATTTTTGA
AATGAAAGAGCAAGGATTGGTGGAAGTTCAAAATCCTTCCGAAGCCTTTTTAGAAGAACGAATTGATGGAGCGACGGGCT
CAGCAATCGTTGTGTCGATGGAAGGAACGAGACCAATCTTGGTTGAGGTTCAGTCTTTGGTAACACCTACCATGTTTGGA
AATGCGAAGAGAACGGCAACTGGACTAGATTATAATCGCGTATCATTAATTATGGCAGTTCTTGAAAAACGTGCAGGTCT
ATTATTACAAAACCAAGATGCATACTTAAAAGCAGCGGGAGGGGTCAAATTGGATGAGCCGGCGATTGATTTGGCTATAG
CGGTTGCGTTAGCCTCAAGCTACAAAGAAAAAGGAACTAAACCAACAGAATGTTTCGTCGGCGAAATAGGCTTAACCGGT
GAAATTCGTCGTGTCAATCGGATTGAGCAACGTGTCGTTGAAGCTCAAAAATTAGGTTTTAAAAAAATGTACCTTCCAAA
AAATAATCTGCAAGGGTGGGAAGCTCCTAAAGGAATTGAGATTGTAGGAGTTGCCACATTAGGAGAAGCGTTACATAAAA
TTTTTGAAAAATCGGAAGCGGCATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-32)

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

73.626

98.485

0.725
  radA Streptococcus pneumoniae Rx1

73.626

98.485

0.725
  radA Streptococcus pneumoniae D39

73.626

98.485

0.725
  radA Streptococcus pneumoniae R6

73.626

98.485

0.725
  radA Streptococcus pneumoniae TIGR4

73.626

98.485

0.725
  radA Streptococcus mitis SK321

73.407

98.485

0.723
  radA Bacillus subtilis subsp. subtilis str. 168

66.593

97.835

0.652