Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A6U93_RS00045 Genome accession   NZ_CP123838
Coordinates   7689..9239 (-) Length   516 a.a.
NCBI ID   WP_025592254.1    Uniprot ID   A0AA44FA84
Organism   Agrobacterium tumefaciens strain 15-1187-1-2a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2689..14239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6U93_RS00015 (A6U93_00015) rdgB 2843..3487 (-) 645 WP_065659817.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  A6U93_RS00020 (A6U93_00020) rph 3505..4221 (-) 717 WP_003514752.1 ribonuclease PH -
  A6U93_RS00025 (A6U93_00025) hrcA 4358..5449 (+) 1092 WP_003514754.1 heat-inducible transcriptional repressor HrcA -
  A6U93_RS00030 (A6U93_00030) grpE 5563..6198 (+) 636 WP_003514756.1 nucleotide exchange factor GrpE -
  A6U93_RS00035 (A6U93_00035) ptsN 6329..6790 (-) 462 WP_003493488.1 PTS IIA-like nitrogen regulatory protein PtsN -
  A6U93_RS00040 (A6U93_00040) hpf 6873..7454 (-) 582 WP_003514759.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A6U93_RS00045 (A6U93_00045) rpoN/rpoN1 7689..9239 (-) 1551 WP_025592254.1 RNA polymerase factor sigma-54 Machinery gene
  A6U93_RS00050 (A6U93_00050) lptB 9393..10202 (-) 810 WP_003514762.1 LPS export ABC transporter ATP-binding protein -
  A6U93_RS00055 (A6U93_00055) - 10212..10778 (-) 567 WP_003514763.1 LptA/OstA family protein -
  A6U93_RS00060 (A6U93_00060) lptC 10792..11457 (-) 666 WP_003514764.1 LPS export ABC transporter periplasmic protein LptC -
  A6U93_RS00065 (A6U93_00065) sppA 11707..12660 (+) 954 WP_013635364.1 signal peptide peptidase SppA -
  A6U93_RS00070 (A6U93_00070) - 12687..12989 (+) 303 WP_003493505.1 integration host factor subunit beta -
  A6U93_RS00075 (A6U93_00075) - 13055..13396 (+) 342 WP_003514766.1 LapA family protein -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 57096.23 Da        Isoelectric Point: 4.4268

>NTDB_id=752713 A6U93_RS00045 WP_025592254.1 7689..9239(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain 15-1187-1-2a]
MALSASLLLRQNQSLVMTPQLMQSIQLLQMTHFELTQFIAQEVERNPLLEIAANDGDLSNDTPADAENFGDADSEGSDKS
APVTADSDDWYGDRAANLGEQLDTSFENVFPDDTEPRKPDAPELAGQWKSMPGQESGESYDLDDFVAAKQTLSDHLNQQL
PLAISSVEDRMIADALIGQLDETGYIAVDAVEDVAERLGAAPSAVERILETLQGFDPPGVFSRSLSECLAIQLAQKDRLD
PAMRAFIDNLELLAKRDFASLKKLCGVDEEDLLDMLAEIRTLNPRPGAGYDSTVSETIVPDIIIRPSSAGGWLVEINPET
LPRVLINQTYFAEVSRHKSREGEDQDFLSECMQTAHWLTRSLDQRARTIMKVATEIVRQQDAFLVNGVDHLRPLNLKTVA
DAIKMHESTVSRVTSKKYMLTPRGLFELKYFFSVSISSVEGGDSHSAEAVRHRIKAMIAQEAADAVLSDDDIVDNLKKSG
IDLARRTVAKYREAMNIPSSVQRRREKKALARLSAF

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=752713 A6U93_RS00045 WP_025592254.1 7689..9239(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain 15-1187-1-2a]
ATGGCATTATCTGCCAGCCTTTTGCTGCGTCAAAACCAGTCTCTCGTGATGACACCGCAACTGATGCAGTCCATCCAGCT
GCTTCAGATGACGCATTTCGAACTGACGCAGTTCATCGCGCAGGAGGTGGAGCGCAATCCTTTGCTTGAAATTGCGGCAA
ACGACGGCGATTTGAGCAACGATACCCCTGCTGATGCGGAGAATTTCGGCGATGCCGACAGCGAAGGGTCCGACAAGTCC
GCGCCGGTAACGGCCGACAGCGACGACTGGTACGGCGATCGCGCGGCAAATCTCGGCGAACAGCTCGACACCAGCTTCGA
AAATGTCTTTCCCGACGATACCGAACCCAGAAAGCCCGACGCACCCGAACTGGCGGGGCAGTGGAAATCCATGCCCGGGC
AGGAGTCAGGCGAGAGTTACGATCTCGACGACTTCGTTGCCGCGAAACAGACCCTCAGCGACCATCTCAATCAGCAATTG
CCTCTCGCCATATCCTCTGTGGAAGACAGGATGATCGCCGATGCGCTGATCGGCCAGCTCGACGAGACCGGATATATCGC
CGTAGATGCCGTCGAAGACGTCGCTGAACGGCTGGGGGCGGCGCCTTCGGCAGTGGAGCGCATTCTGGAGACGCTTCAGG
GCTTTGATCCTCCCGGCGTGTTTTCCCGCTCGCTGAGCGAATGTCTGGCGATCCAGCTTGCCCAGAAGGACAGGCTTGAT
CCGGCAATGCGGGCTTTTATCGACAATCTGGAGCTTCTGGCGAAACGGGATTTCGCTTCTCTAAAAAAACTCTGCGGCGT
GGATGAGGAAGACCTTCTCGACATGCTGGCGGAAATCCGCACGCTCAATCCGCGTCCGGGCGCTGGTTACGATTCGACCG
TTTCCGAGACAATCGTTCCTGATATCATCATTCGCCCCTCCTCTGCCGGCGGCTGGCTTGTGGAAATCAACCCCGAGACC
CTGCCGCGCGTGCTCATCAACCAGACCTATTTCGCAGAGGTTTCAAGGCACAAGTCGCGCGAGGGAGAGGATCAGGATTT
CCTTTCCGAATGCATGCAGACGGCCCATTGGCTGACCCGCAGCCTCGACCAGCGCGCCAGAACGATCATGAAGGTGGCGA
CCGAAATCGTGCGCCAGCAGGACGCCTTTCTCGTCAATGGCGTGGACCACCTTCGCCCGCTGAACCTCAAGACGGTGGCC
GACGCTATCAAGATGCACGAATCCACCGTCAGCCGGGTGACGTCGAAAAAATACATGCTGACGCCGCGCGGCCTGTTCGA
GCTGAAATATTTCTTCAGTGTTTCCATCAGCTCGGTGGAAGGCGGTGACAGCCATTCGGCAGAAGCGGTGCGTCATCGCA
TCAAGGCGATGATCGCGCAGGAGGCCGCCGACGCAGTGCTTTCCGACGACGATATCGTCGATAATCTGAAAAAGAGTGGT
ATCGATCTTGCCCGCCGGACCGTGGCCAAATATCGCGAGGCGATGAACATCCCATCCTCCGTTCAGAGACGGCGCGAGAA
GAAGGCCCTGGCCAGACTGTCGGCGTTCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-505)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-51)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AA44FA84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

36.243

100

0.37