Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OKW88_RS06580 Genome accession   NZ_CP110105
Coordinates   1311400..1311810 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain CF03     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1306400..1316810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW88_RS06550 (OKW88_06535) - 1306487..1306627 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  OKW88_RS06555 (OKW88_06540) yqeF 1306933..1307664 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  OKW88_RS06560 (OKW88_06545) cwlH 1307916..1308668 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  OKW88_RS06565 (OKW88_06550) yqeD 1308855..1309481 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  OKW88_RS06570 (OKW88_06555) gnd 1309500..1310393 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  OKW88_RS06575 (OKW88_06560) yqeB 1310645..1311367 (+) 723 WP_010886572.1 hypothetical protein -
  OKW88_RS06580 (OKW88_06565) nucA/comI 1311400..1311810 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  OKW88_RS06585 (OKW88_06570) sigK 1312006..1312734 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  OKW88_RS06590 (OKW88_06575) - 1312734..1312850 (+) 117 WP_122894874.1 hypothetical protein -
  OKW88_RS06595 (OKW88_06580) - 1312816..1313012 (-) 197 Protein_1314 recombinase family protein -
  OKW88_RS06600 (OKW88_06585) - 1312970..1313134 (-) 165 WP_080265384.1 hypothetical protein -
  OKW88_RS06605 (OKW88_06590) - 1313567..1314160 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  OKW88_RS06610 (OKW88_06595) - 1314318..1315313 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  OKW88_RS06615 (OKW88_06600) - 1315641..1315874 (+) 234 Protein_1318 helix-turn-helix domain-containing protein -
  OKW88_RS06620 (OKW88_06605) arsC 1316085..1316504 (-) 420 WP_029318100.1 arsenate reductase (thioredoxin) -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=752271 OKW88_RS06580 WP_009967785.1 1311400..1311810(-) (nucA/comI) [Bacillus subtilis strain CF03]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=752271 OKW88_RS06580 WP_009967785.1 1311400..1311810(-) (nucA/comI) [Bacillus subtilis strain CF03]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529