Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OU418_RS13590 Genome accession   NZ_CP123621
Coordinates   2602955..2603365 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain RLI2019     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2597955..2608365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU418_RS13560 (OU418_13560) - 2598042..2598182 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  OU418_RS13565 (OU418_13565) yqeF 2598487..2599218 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  OU418_RS13570 (OU418_13570) cwlH 2599470..2600222 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  OU418_RS13575 (OU418_13575) yqeD 2600409..2601035 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  OU418_RS13580 (OU418_13580) gnd 2601055..2601948 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  OU418_RS13585 (OU418_13585) yqeB 2602200..2602922 (+) 723 WP_032726205.1 hypothetical protein -
  OU418_RS13590 (OU418_13590) nucA/comI 2602955..2603365 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  OU418_RS13595 (OU418_13595) - 2603561..2603911 (+) 351 Protein_2637 sigma-70 family RNA polymerase sigma factor -
  OU418_RS13600 (OU418_13600) spoIVCA 2603939..2605399 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  OU418_RS13605 (OU418_13605) - 2605357..2605535 (-) 179 Protein_2639 hypothetical protein -
  OU418_RS13610 (OU418_13610) arsC 2605890..2606309 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  OU418_RS13615 (OU418_13615) acr3 2606321..2607361 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  OU418_RS13620 (OU418_13620) arsK 2607384..2607824 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  OU418_RS13625 (OU418_13625) arsR 2607883..2608200 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=751453 OU418_RS13590 WP_009967785.1 2602955..2603365(-) (nucA/comI) [Bacillus subtilis strain RLI2019]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=751453 OU418_RS13590 WP_009967785.1 2602955..2603365(-) (nucA/comI) [Bacillus subtilis strain RLI2019]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529