Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MPB2EB_RS07545 Genome accession   NZ_AP021872
Coordinates   1698301..1699326 (+) Length   341 a.a.
NCBI ID   WP_185181713.1    Uniprot ID   -
Organism   Mycoavidus sp. B2-EB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1693301..1704326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPB2EB_RS07520 (MPB2EB_1490) - 1693763..1694020 (-) 258 WP_185181709.1 YfhL family 4Fe-4S dicluster ferredoxin -
  MPB2EB_RS07525 (MPB2EB_1491) coaD 1694043..1694552 (-) 510 WP_185181710.1 pantetheine-phosphate adenylyltransferase -
  MPB2EB_RS07530 (MPB2EB_1492) rsmD 1694549..1695241 (-) 693 WP_185181711.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  MPB2EB_RS07535 (MPB2EB_1493) - 1695262..1696605 (-) 1344 WP_185181712.1 pitrilysin family protein -
  MPB2EB_RS07540 (MPB2EB_1494) - 1696620..1698032 (-) 1413 WP_185182704.1 pitrilysin family protein -
  MPB2EB_RS07545 (MPB2EB_1495) pilA 1698301..1699326 (+) 1026 WP_185181713.1 signal recognition particle-docking protein FtsY Machinery gene
  MPB2EB_RS07550 (MPB2EB_1496) kdsB 1699413..1700234 (-) 822 WP_185181714.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  MPB2EB_RS07555 (MPB2EB_1497) - 1700656..1701354 (-) 699 WP_185181715.1 fumarylacetoacetate hydrolase family protein -
  MPB2EB_RS07560 (MPB2EB_1498) rpoH 1701646..1702560 (+) 915 WP_185181716.1 RNA polymerase sigma factor RpoH -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37000.54 Da        Isoelectric Point: 6.9837

>NTDB_id=75138 MPB2EB_RS07545 WP_185181713.1 1698301..1699326(+) (pilA) [Mycoavidus sp. B2-EB]
MFSFFKKLHKSSTSAPEPAEAEHKTKNDTQDKNPSAPALTPDRPTSWVNRLKSGLAKTRNNFTNLFTNVKIDEDFFEELE
AALLMADTGLEASQFLLNALRQKVLAERLNDGEAVKAALRSLLADLLKPLEHTLVLDRAQPLVMMITGVNGAGKTTSIGK
LAQHLQRHGQSVLLAAGDTFRAAAHEQLALWGERNQINVISQQSGDPAAVIFDALNAARARKLDILMADTAGRLPTQLHL
MEELKKIKRVIAKAMADAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPIPVYFIGV
GEHIDDLQPFCAEEFANALLN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=75138 MPB2EB_RS07545 WP_185181713.1 1698301..1699326(+) (pilA) [Mycoavidus sp. B2-EB]
ATGTTTAGCTTCTTCAAAAAGCTTCATAAGAGCTCAACATCAGCCCCCGAACCTGCTGAGGCAGAGCATAAAACAAAAAA
CGACACTCAGGATAAAAATCCTTCCGCTCCAGCGCTGACTCCAGATCGTCCAACCTCCTGGGTCAACCGGCTAAAATCTG
GTTTGGCGAAAACCCGCAACAACTTTACAAACCTTTTTACCAACGTCAAGATCGATGAGGACTTTTTTGAAGAACTGGAA
GCTGCGCTTTTAATGGCCGACACGGGGCTTGAGGCAAGCCAATTCTTGCTGAATGCTCTGCGCCAAAAAGTCCTTGCGGA
ACGGCTTAATGACGGTGAAGCCGTTAAAGCAGCACTGCGCTCCTTATTAGCGGATTTATTAAAACCTCTTGAACACACAT
TAGTGCTTGACCGTGCGCAACCCTTAGTGATGATGATCACCGGCGTGAACGGCGCCGGCAAAACCACTAGCATTGGCAAA
CTCGCCCAACATTTACAACGGCATGGACAATCTGTATTGCTTGCCGCGGGCGACACTTTTCGCGCCGCCGCGCACGAACA
GCTTGCGCTATGGGGGGAACGTAATCAGATTAATGTCATCTCACAGCAAAGCGGAGACCCCGCCGCAGTCATTTTCGACG
CCCTCAACGCGGCACGCGCACGTAAGCTAGACATCCTGATGGCGGACACCGCCGGGCGCCTGCCAACGCAATTGCATTTG
ATGGAGGAGTTGAAAAAAATCAAACGCGTGATTGCTAAAGCCATGGCTGATGCTCCGCATGAGGTACTGCTAGTGATTGA
CGCAAACACTGGGCAGAATGCCTTGGCGCAAGTCAAGGCTTTTGATGATGCGCTTGGCCTAACCGGATTGATTGTAACCA
AGCTCGATGGCACTGCGAAAGGCGGCATCCTTGCCGCCATTGCGCGACAGCGGCCGATCCCCGTGTACTTCATCGGCGTA
GGGGAACACATTGATGATTTACAGCCATTTTGCGCCGAAGAGTTTGCCAACGCGCTGCTGAACTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(61-121)

SRP54-type protein, GTPase domain (SRP54)

(142-340)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

58.104

95.894

0.557

Multiple sequence alignment