Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MPB2EB_RS03970 Genome accession   NZ_AP021872
Coordinates   880150..881523 (+) Length   457 a.a.
NCBI ID   WP_185182548.1    Uniprot ID   -
Organism   Mycoavidus sp. B2-EB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 875150..886523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPB2EB_RS03950 (MPB2EB_0784) - 875736..876653 (-) 918 WP_185182544.1 Rpn family recombination-promoting nuclease/putative transposase -
  MPB2EB_RS03955 (MPB2EB_0785) thiD 876793..877602 (-) 810 WP_185182545.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  MPB2EB_RS03960 (MPB2EB_0786) lplT 877636..878940 (-) 1305 WP_185182546.1 lysophospholipid transporter LplT -
  MPB2EB_RS03965 (MPB2EB_0787) alr 879079..880143 (+) 1065 WP_185182547.1 alanine racemase -
  MPB2EB_RS03970 (MPB2EB_0788) radA 880150..881523 (+) 1374 WP_185182548.1 DNA repair protein RadA Machinery gene
  MPB2EB_RS03975 (MPB2EB_0789) - 881877..882590 (+) 714 WP_185182549.1 hypothetical protein -
  MPB2EB_RS03980 (MPB2EB_0790) - 882660..885212 (+) 2553 WP_185182550.1 hypothetical protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48739.39 Da        Isoelectric Point: 7.6657

>NTDB_id=75130 MPB2EB_RS03970 WP_185182548.1 880150..881523(+) (radA) [Mycoavidus sp. B2-EB]
MAKKALYVCSACGAQSPKWQGKCFACNAWDTLIETLAETPAAHRYQALTQSTPSQKLAAIEAADVPRFSSGLQEFDRVLG
GGLVAGGVVLIGGDPGIGKSTLLLQSLAALARERRVLYVSGEESGAQIALRAQRLGLVDNATRASELHLLAEIQLEKIQA
AIVAEQPTVAVIDSIQTLYSEALSSAPGSVAQVRECAAQLTRLAKQMGVTIIMVGHVTKEGSLAGPRVLEHIVDTVLYFE
GDTHSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEHRVPGSCVLVTQEGSRPLLVEIQALVDTAHVP
NPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSLRNQPLPNGLVVFGEIGLAG
EIRPCPRGQERLKEAAKLGFSLALIPTANAPKQAIEGLEIIAVDRIEKAIEQIRVRV

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=75130 MPB2EB_RS03970 WP_185182548.1 880150..881523(+) (radA) [Mycoavidus sp. B2-EB]
ATGGCGAAAAAAGCACTTTATGTCTGCAGCGCTTGTGGCGCGCAATCACCTAAATGGCAAGGGAAATGTTTTGCCTGTAA
CGCGTGGGATACGCTGATTGAAACCCTAGCTGAAACGCCCGCGGCTCATCGTTATCAAGCCTTGACGCAGTCAACCCCAA
GTCAGAAACTGGCCGCTATTGAGGCCGCCGATGTGCCGCGCTTTTCAAGCGGCTTACAAGAGTTTGACCGGGTGTTGGGC
GGTGGTTTAGTGGCGGGTGGGGTGGTCCTGATTGGCGGAGACCCGGGCATAGGAAAATCAACATTGTTATTGCAATCACT
TGCCGCCTTAGCCCGTGAGCGCCGGGTATTGTATGTCAGCGGTGAAGAATCAGGTGCGCAAATCGCCTTGCGCGCGCAAC
GTTTAGGTCTGGTGGATAATGCTACACGCGCGAGCGAGCTACATTTACTCGCCGAGATTCAGCTCGAAAAAATCCAAGCA
GCTATTGTGGCTGAACAACCGACCGTAGCGGTGATTGATTCCATCCAAACGCTCTACTCAGAAGCGCTTAGCTCAGCACC
TGGTTCAGTCGCGCAAGTGCGCGAATGTGCGGCACAACTTACGCGCTTAGCCAAACAAATGGGCGTAACCATTATTATGG
TTGGCCACGTCACAAAAGAGGGCAGTTTAGCTGGGCCGCGCGTGCTTGAGCATATTGTGGATACAGTGCTGTATTTCGAG
GGAGATACGCATTCCGCCTACCGGCTCGTGCGCGCTTTTAAAAATCGTTTTGGCGCAGTCAATGAACTCGGCGTCTTTGC
GATGACCGAGCGTGGCTTGCGCGGTGTGGCGAATCCGTCGGCGTTGTTTCTATCGCAGCATGAACATCGCGTGCCAGGTT
CTTGTGTATTAGTGACGCAAGAAGGCTCGCGGCCGTTGTTAGTTGAAATTCAGGCTCTGGTTGACACGGCTCACGTGCCC
AATCCTCGTCGACTCGCCGTTGGGCTTGAGCAAAATCGGCTCGCAATGTTATTGGCGGTGTTACACCGTCACGCAGGTAT
TGCCTGTTTTGATCAAGATGTATTTTTGAACGCTGTTGGCGGAGTTAAAATCTCTGAGCCGGCTGCCGACCTTGCGGTCT
TGCTTGCGATTCAATCCTCCCTGCGCAACCAGCCACTGCCCAATGGTTTAGTTGTATTTGGCGAAATTGGACTCGCTGGC
GAGATTCGTCCTTGCCCACGCGGACAAGAACGTTTGAAAGAGGCGGCAAAATTAGGTTTTTCATTGGCGTTGATTCCAAC
TGCAAATGCCCCTAAACAAGCCATTGAAGGGCTTGAGATTATTGCGGTGGATCGAATTGAAAAAGCCATTGAACAGATAC
GTGTGAGAGTGTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(362-452)

AAA domain (AAA_25)

(63-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50

100

0.505
  radA Streptococcus mitis SK321

46.711

99.781

0.466
  radA Streptococcus mitis NCTC 12261

46.491

99.781

0.464
  radA Streptococcus pneumoniae TIGR4

46.389

100

0.464
  radA Streptococcus pneumoniae R6

46.389

100

0.464
  radA Streptococcus pneumoniae Rx1

46.389

100

0.464
  radA Streptococcus pneumoniae D39

46.389

100

0.464

Multiple sequence alignment