Detailed information NTG0750348

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: OK229_RS25470
• Genome accession: NZ_CP109872
• Coordinates: 4876428..4877777 (-)
• Length: 449 a.a.
• NCBI ID: WP_000879417.1
• Uniprot ID: A0AAE9TF02
• Organism: Bacillus cereus strain BC38B
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4871428..4882777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK229_RS25450 (OK229_08330)	secA	4871749..4874256 (-)	2508	WP_000579384.1	preprotein translocase subunit SecA	-
OK229_RS25455 (OK229_08335)	raiA	4874533..4875075 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
OK229_RS25460 (OK229_08340)	cspC	4875400..4875597 (-)	198	WP_001990088.1	cold shock protein CspC	-
OK229_RS25465 (OK229_08345)	comFC	4875724..4876428 (-)	705	WP_002157779.1	comF operon protein ComFC	-
OK229_RS25470 (OK229_08350)	comFA	4876428..4877777 (-)	1350	WP_000879417.1	ATP-dependent helicase ComFA	Machinery gene
OK229_RS25475 (OK229_08355)	-	4877905..4879230 (-)	1326	WP_264459507.1	C40 family peptidase	-
OK229_RS25480 (OK229_08360)	-	4879483..4879797 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
OK229_RS25485 (OK229_08365)	-	4879971..4880813 (-)	843	WP_000844766.1	DegV family protein	-
OK229_RS25490 (OK229_08370)	-	4881053..4881688 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51233.89 Da
• Isoelectric Point: 9.5047

>NTDB_id=750348 OK229_RS25470 WP_000879417.1 4876428..4877777(-) (comFA) [Bacillus cereus strain BC38B]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=750348 OK229_RS25470 WP_000879417.1 4876428..4877777(-) (comFA) [Bacillus cereus strain BC38B]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACGGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGCTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCATTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.704	88.641	0.361