Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   OHU65_RS22690 Genome accession   NZ_CP109859
Coordinates   4336362..4337414 (-) Length   350 a.a.
NCBI ID   WP_221784571.1    Uniprot ID   -
Organism   Priestia megaterium strain 207     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4331362..4342414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU65_RS22650 (OHU65_22650) - 4332377..4333201 (+) 825 WP_221784567.1 YqhG family protein -
  OHU65_RS22655 (OHU65_22655) - 4333226..4333411 (-) 186 WP_013059170.1 YqzE family protein -
  OHU65_RS22660 (OHU65_22660) comGG 4333455..4333829 (-) 375 WP_221784568.1 competence type IV pilus minor pilin ComGG -
  OHU65_RS22665 (OHU65_22665) comGF 4333819..4334256 (-) 438 WP_264408568.1 competence type IV pilus minor pilin ComGF -
  OHU65_RS22670 (OHU65_22670) - 4334253..4334591 (-) 339 WP_028410645.1 type II secretion system protein -
  OHU65_RS22675 (OHU65_22675) comGD 4334575..4335057 (-) 483 WP_223260330.1 competence type IV pilus minor pilin ComGD -
  OHU65_RS22680 (OHU65_22680) comGC 4334999..4335310 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  OHU65_RS22685 (OHU65_22685) comGB 4335325..4336371 (-) 1047 WP_221784570.1 competence type IV pilus assembly protein ComGB -
  OHU65_RS22690 (OHU65_22690) comGA 4336362..4337414 (-) 1053 WP_221784571.1 competence type IV pilus ATPase ComGA Machinery gene
  OHU65_RS22695 (OHU65_22695) - 4337684..4338067 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  OHU65_RS22700 (OHU65_22700) - 4338329..4338565 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  OHU65_RS22705 (OHU65_22705) - 4338603..4339235 (-) 633 WP_221784572.1 MBL fold metallo-hydrolase -
  OHU65_RS22710 (OHU65_22710) - 4339444..4339620 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  OHU65_RS22715 (OHU65_22715) - 4339656..4340558 (-) 903 WP_221784573.1 M14 family metallocarboxypeptidase -
  OHU65_RS22720 (OHU65_22720) - 4340670..4341638 (-) 969 WP_013059183.1 ROK family glucokinase -
  OHU65_RS22725 (OHU65_22725) - 4341641..4341847 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39598.94 Da        Isoelectric Point: 9.0586

>NTDB_id=750279 OHU65_RS22690 WP_221784571.1 4336362..4337414(-) (comGA) [Priestia megaterium strain 207]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELSLKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=750279 OHU65_RS22690 WP_221784571.1 4336362..4337414(-) (comGA) [Priestia megaterium strain 207]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTAGTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCACTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGACT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACCACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGGAATGTTATTACATTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGCGCAGGGATTACGTATACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACCGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACAATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGGGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCGCAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCTGCTTGTAAGAAAATGAGGA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGCTATTTATATCCGCATTCGCTAGA
TGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586