Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OHU65_RS19545 Genome accession   NZ_CP109859
Coordinates   3719545..3719919 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain 207     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3714545..3724919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU65_RS19525 (OHU65_19525) - 3714882..3715553 (-) 672 WP_221812524.1 response regulator transcription factor -
  OHU65_RS19530 (OHU65_19530) - 3715879..3716697 (+) 819 WP_221783690.1 squalene/phytoene synthase family protein -
  OHU65_RS19535 (OHU65_19535) - 3716733..3718682 (-) 1950 WP_221783688.1 FUSC family protein -
  OHU65_RS19540 (OHU65_19540) - 3718839..3719099 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  OHU65_RS19545 (OHU65_19545) nucA/comI 3719545..3719919 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  OHU65_RS19550 (OHU65_19550) comJ 3719955..3720362 (+) 408 WP_221783686.1 competence protein ComJ -
  OHU65_RS19555 (OHU65_19555) helD 3720396..3722714 (-) 2319 WP_221812525.1 RNA polymerase recycling motor HelD -
  OHU65_RS19560 (OHU65_19560) - 3722960..3723085 (-) 126 WP_016765178.1 hypothetical protein -
  OHU65_RS19565 (OHU65_19565) - 3723225..3723740 (-) 516 WP_264408463.1 enoyl-CoA hydratase/isomerase family protein -
  OHU65_RS19570 (OHU65_19570) - 3723983..3724351 (+) 369 WP_060747183.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=750273 OHU65_RS19545 WP_223269005.1 3719545..3719919(+) (nucA/comI) [Priestia megaterium strain 207]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=750273 OHU65_RS19545 WP_223269005.1 3719545..3719919(+) (nucA/comI) [Priestia megaterium strain 207]
ATGGGACATGCGGCTTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTATGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCAATTGCCGCTGGGCACTCTTCCGTTTGCACGATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCCGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTGCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645