Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OHU65_RS16495 Genome accession   NZ_CP109859
Coordinates   3218686..3219108 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain 207     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3213686..3224108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU65_RS16470 (OHU65_16470) - 3214012..3214845 (-) 834 WP_221784137.1 AraC family transcriptional regulator -
  OHU65_RS16475 (OHU65_16475) - 3215068..3215889 (+) 822 WP_221784135.1 sensor histidine kinase -
  OHU65_RS16480 (OHU65_16480) - 3215890..3216531 (+) 642 WP_221784134.1 response regulator transcription factor -
  OHU65_RS16485 (OHU65_16485) - 3216669..3218051 (+) 1383 WP_221784132.1 PepSY domain-containing protein -
  OHU65_RS16490 (OHU65_16490) - 3218081..3218545 (+) 465 WP_264407984.1 FixH family protein -
  OHU65_RS16495 (OHU65_16495) nucA/comI 3218686..3219108 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  OHU65_RS16500 (OHU65_16500) - 3219249..3219602 (+) 354 WP_223272376.1 DUF1516 family protein -
  OHU65_RS16505 (OHU65_16505) - 3219643..3220236 (-) 594 WP_013057951.1 hypothetical protein -
  OHU65_RS16510 (OHU65_16510) - 3220390..3221046 (-) 657 WP_221784130.1 TetR/AcrR family transcriptional regulator -
  OHU65_RS16515 (OHU65_16515) - 3221070..3222347 (-) 1278 WP_221784128.1 glycosyltransferase -
  OHU65_RS16520 (OHU65_16520) - 3222646..3223191 (+) 546 WP_221784126.1 GNAT family N-acetyltransferase -
  OHU65_RS16525 (OHU65_16525) - 3223466..3223969 (-) 504 WP_221784124.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=750269 OHU65_RS16495 WP_013057949.1 3218686..3219108(+) (nucA/comI) [Priestia megaterium strain 207]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=750269 OHU65_RS16495 WP_013057949.1 3218686..3219108(+) (nucA/comI) [Priestia megaterium strain 207]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATTGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGAGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCAGATAT
TGAATATGTAAAGCCTTCAGACAACCGCGGCGCCGGTTCTTGGATTTCACATCAAGTAGATGAGTTTCCAGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507