Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OHU65_RS00535 Genome accession   NZ_CP109859
Coordinates   99733..101109 (+) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia megaterium strain 207     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94733..106109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU65_RS00515 (OHU65_00515) - 95025..95486 (+) 462 WP_013054895.1 CtsR family transcriptional regulator -
  OHU65_RS00520 (OHU65_00520) - 95528..96070 (+) 543 WP_025753183.1 UvrB/UvrC motif-containing protein -
  OHU65_RS00525 (OHU65_00525) - 96074..97147 (+) 1074 WP_013054897.1 protein arginine kinase -
  OHU65_RS00530 (OHU65_00530) clpC 97168..99609 (+) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  OHU65_RS00535 (OHU65_00535) radA 99733..101109 (+) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  OHU65_RS00540 (OHU65_00540) disA 101113..102192 (+) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  OHU65_RS00545 (OHU65_00545) - 102340..103437 (+) 1098 WP_013054901.1 PIN/TRAM domain-containing protein -
  OHU65_RS00550 (OHU65_00550) ispD 103463..104164 (+) 702 WP_221785578.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  OHU65_RS00555 (OHU65_00555) ispF 104161..104640 (+) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=750246 OHU65_RS00535 WP_013054899.1 99733..101109(+) (radA) [Priestia megaterium strain 207]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=750246 OHU65_RS00535 WP_013054899.1 99733..101109(+) (radA) [Priestia megaterium strain 207]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCACTGCGGATATGAATCAGCAAAATGGATGGGGAAATGTCCCGGATG
CGGAAGCTGGAATTCCATGGTAGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTCCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGAATTTTTACACCTTCTGCAGAGTTA
AATCGTGTATTAGGTGGAGGTATTGTGAGAGGCTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAATTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGACAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCATGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGAATTGCAATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCAGGACCAAGACTTCTTGAACATATGGTGGATACGGTTCTTTATTTT
GAAGGAGAGCGCCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGATTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GATCGGTTGTTGTCGCTTCAATGGAAGGGACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCTACGGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCGTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGTGAAGTACGCCGAGTGTCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATCCC
CGAGAAGAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624