Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N4686_RS02545 Genome accession   NZ_CP123266
Coordinates   523914..525347 (-) Length   477 a.a.
NCBI ID   WP_000809036.1    Uniprot ID   -
Organism   Escherichia coli strain YZLc23-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518914..530347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4686_RS02510 (N4686_002510) elbB 519605..520258 (+) 654 WP_001297560.1 isoprenoid biosynthesis glyoxalase ElbB -
  N4686_RS02515 (N4686_002515) mtgA 520255..520983 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N4686_RS02520 (N4686_002520) yrbL 520980..521612 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  N4686_RS02525 (N4686_002525) npr 521826..522098 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  N4686_RS02530 (N4686_002530) rapZ 522095..522949 (-) 855 WP_000243741.1 RNase adapter RapZ -
  N4686_RS02535 (N4686_002535) ptsN 522995..523486 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N4686_RS02540 (N4686_002540) hpf 523604..523891 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  N4686_RS02545 (N4686_002545) rpoN/rpoN1 523914..525347 (-) 1434 WP_000809036.1 RNA polymerase factor sigma-54 Machinery gene
  N4686_RS02550 (N4686_002550) lptB 525395..526120 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  N4686_RS02555 (N4686_002555) lptA 526127..526684 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N4686_RS02560 (N4686_002560) lptC 526653..527228 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  N4686_RS02565 (N4686_002565) kdsC 527225..527791 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N4686_RS02570 (N4686_002570) kdsD 527812..528798 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  N4686_RS02575 (N4686_002575) yrbG 528812..529789 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54007.82 Da        Isoelectric Point: 4.3639

>NTDB_id=750008 N4686_RS02545 WP_000809036.1 523914..525347(-) (rpoN/rpoN1) [Escherichia coli strain YZLc23-1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=750008 N4686_RS02545 WP_000809036.1 523914..525347(-) (rpoN/rpoN1) [Escherichia coli strain YZLc23-1]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGATCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465