Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   MRSL_RS00400 Genome accession   NZ_AP021848
Coordinates   85545..87173 (-) Length   542 a.a.
NCBI ID   WP_172412330.1    Uniprot ID   -
Organism   Staphylococcus lugdunensis strain JICS135     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 80545..92173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRSL_RS00375 (MRSL_00740) gtfA 80755..82263 (+) 1509 WP_001551920.1 accessory Sec system glycosyltransferase GtfA -
  MRSL_RS00380 (MRSL_00750) gtfB 82256..83575 (+) 1320 WP_002468074.1 accessory Sec system glycosylation chaperone GtfB -
  MRSL_RS00385 (MRSL_00760) - 83756..84259 (-) 504 WP_058649860.1 DUF1643 domain-containing protein -
  MRSL_RS00390 (MRSL_00770) - 84274..84588 (-) 315 WP_058649861.1 DUF960 family protein -
  MRSL_RS00395 (MRSL_00780) - 84681..85022 (-) 342 WP_058649862.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MRSL_RS00400 (MRSL_00790) ccrB 85545..87173 (-) 1629 WP_172412330.1 cassette chromosome recombinase CcrB Machinery gene
  MRSL_RS00405 (MRSL_00800) ccrA 87195..88544 (-) 1350 WP_001573628.1 cassette chromosome recombinase CcrA Machinery gene
  MRSL_RS00410 (MRSL_00810) cch1 88778..90565 (-) 1788 WP_058649864.1 cassette chromosome replicative helicase -
  MRSL_RS00415 (MRSL_00820) ssb 90565..90861 (-) 297 WP_002454339.1 cassette chromosome ssDNA-binding protein -
  MRSL_RS00420 (MRSL_00830) - 91054..92100 (-) 1047 WP_001042023.1 hypothetical protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62660.88 Da        Isoelectric Point: 9.8616

>NTDB_id=74973 MRSL_RS00400 WP_172412330.1 85545..87173(-) (ccrB) [Staphylococcus lugdunensis strain JICS135]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRILKDAKDGYIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPNSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADAIEDYVMKQILEIVKSDKVIQQVVARVNQENQVDCASLHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYEKKLNDITNQINQLKNQQNEDMTLFNAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKHFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=74973 MRSL_RS00400 WP_172412330.1 85545..87173(-) (ccrB) [Staphylococcus lugdunensis strain JICS135]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTTTCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACGTATATTGAAGGATGCGAAAGATGGCTATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAAATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAATGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGTAAC
TTGCCGCTGGGCTATGACAAAATACCTAATAGTAAACACGAACTGATGATTAATCAACATGAAGCTAATATTGTGAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTAAATCACAAAGGCTATGTCACTAAAA
AGGGTAAACCTTTTAGTATTAGTTCTATCACATATATATTAGCTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCTCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACACTTAAAGAC
GGGACCAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGCTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGCGATTGAAGATTATGTGATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAAGTCATTCAACAGGTTG
TAGCACGTGTTAATCAAGAAAATCAAGTTGATTGTGCGTCACTTCATCACGATATCGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGAAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATATGACTT
TATTTAATGCCAAAGAAATTAGTAAACTATTACAACACATCTTTCATGATATTAAGCACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACATTTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTGTTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterProScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

97.786

100

0.978

Multiple sequence alignment