Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OHZ10_RS15175 Genome accession   NZ_CP109821
Coordinates   3232230..3233735 (+) Length   501 a.a.
NCBI ID   WP_059233171.1    Uniprot ID   A0A9Q9SP11
Organism   Burkholderia arboris strain PN-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3227230..3238735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHZ10_RS15150 (OHZ10_15125) kdsD 3228474..3229457 (+) 984 WP_059233175.1 arabinose 5-phosphate isomerase KdsD -
  OHZ10_RS15155 (OHZ10_15130) - 3229457..3229993 (+) 537 WP_174994104.1 HAD family hydrolase -
  OHZ10_RS15160 (OHZ10_15135) lptC 3229997..3230611 (+) 615 WP_059233174.1 LPS export ABC transporter periplasmic protein LptC -
  OHZ10_RS15165 (OHZ10_15140) lptA 3230632..3231297 (+) 666 WP_059233173.1 lipopolysaccharide transport periplasmic protein LptA -
  OHZ10_RS15170 (OHZ10_15145) lptB 3231294..3232070 (+) 777 WP_059233172.1 LPS export ABC transporter ATP-binding protein -
  OHZ10_RS15175 (OHZ10_15150) rpoN/rpoN1 3232230..3233735 (+) 1506 WP_059233171.1 RNA polymerase factor sigma-54 Machinery gene
  OHZ10_RS15180 (OHZ10_15155) raiA 3233893..3234252 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  OHZ10_RS15185 (OHZ10_15160) - 3234448..3234960 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  OHZ10_RS15190 (OHZ10_15165) hprK 3235078..3236046 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  OHZ10_RS15195 (OHZ10_15170) rapZ 3236122..3237030 (+) 909 WP_059233170.1 RNase adapter RapZ -
  OHZ10_RS15200 (OHZ10_15175) - 3237058..3237693 (+) 636 WP_340624486.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54849.99 Da        Isoelectric Point: 4.9339

>NTDB_id=749626 OHZ10_RS15175 WP_059233171.1 3232230..3233735(+) (rpoN/rpoN1) [Burkholderia arboris strain PN-1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQHNEPAS
TNGSSTSGDRAERDEPAGADEYDSYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=749626 OHZ10_RS15175 WP_059233171.1 3232230..3233735(+) (rpoN/rpoN1) [Burkholderia arboris strain PN-1]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAACCCGTTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGTTGATCGCGCAGACGCCCCCCGGGCAGCATAACGAGCCGGCCTCG
ACGAACGGCAGCAGCACGTCCGGCGATCGCGCGGAGCGCGACGAGCCGGCTGGCGCCGACGAATACGACAGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGATGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGC
CCGGCGTCGGCGCACGCTCGGCGTCGGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCGGCCCGCGATTTCACGCGGTTGCGCAAGCACCTGAAGGCAAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCATGCCG
CGCCTGCGGATCAACAACCTGTACGCAAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGGTGGCTGATCAAGAATATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCACGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691