Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   OH128_RS03415 Genome accession   NZ_CP109813
Coordinates   679476..680420 (-) Length   314 a.a.
NCBI ID   WP_052779190.1    Uniprot ID   -
Organism   Campylobacter coli strain CC20JX12     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 668647..695501 679476..680420 within 0


Gene organization within MGE regions


Location: 668647..695501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH128_RS03365 (OH128_03365) - 668647..670203 (-) 1557 WP_264378409.1 DNA polymerase III subunit gamma/tau -
  OH128_RS03370 (OH128_03370) rho 670206..671504 (-) 1299 WP_002780613.1 transcription termination factor Rho -
  OH128_RS03375 (OH128_03375) - 671617..673980 (+) 2364 WP_264378410.1 heavy metal translocating P-type ATPase -
  OH128_RS03380 (OH128_03380) - 673973..674173 (+) 201 WP_002777240.1 cbb3-type cytochrome oxidase assembly protein -
  OH128_RS03385 (OH128_03385) - 674203..674502 (-) 300 WP_002777238.1 cytochrome c -
  OH128_RS03390 (OH128_03390) - 674579..675142 (+) 564 WP_002787747.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  OH128_RS03395 (OH128_03395) rfaD 675139..676092 (+) 954 WP_264378411.1 ADP-glyceromanno-heptose 6-epimerase -
  OH128_RS03400 (OH128_03400) rfaE1 676085..677470 (+) 1386 WP_264378412.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  OH128_RS03405 (OH128_03405) gmhA 677467..678027 (+) 561 WP_052783332.1 D-sedoheptulose 7-phosphate isomerase -
  OH128_RS03410 (OH128_03410) - 678039..679493 (+) 1455 WP_002791237.1 hypothetical protein -
  OH128_RS03415 (OH128_03415) waaF 679476..680420 (-) 945 WP_052779190.1 lipopolysaccharide heptosyltransferase II Regulator
  OH128_RS03420 (OH128_03420) - 680479..681294 (+) 816 WP_052779189.1 glycosyltransferase family 2 protein -
  OH128_RS03425 (OH128_03425) - 681313..681729 (-) 417 WP_002796922.1 adenylyltransferase/cytidyltransferase family protein -
  OH128_RS03430 (OH128_03430) - 681732..682781 (-) 1050 WP_002804034.1 asparagine synthase C-terminal domain-containing protein -
  OH128_RS03435 (OH128_03435) - 682778..684082 (-) 1305 WP_052783333.1 hypothetical protein -
  OH128_RS03440 (OH128_03440) - 684210..685409 (+) 1200 WP_002797725.1 glycosyltransferase family 8 protein -
  OH128_RS03445 (OH128_03445) - 685406..686458 (-) 1053 WP_002791222.1 glycosyltransferase family 4 protein -
  OH128_RS03450 (OH128_03450) - 686455..688008 (-) 1554 WP_264378413.1 glycosyltransferase -
  OH128_RS03455 (OH128_03455) - 688005..688892 (-) 888 WP_002797723.1 lipid A biosynthesis lauroyl acyltransferase -
  OH128_RS03460 (OH128_03460) waaC 688885..689913 (-) 1029 WP_002786299.1 lipopolysaccharide heptosyltransferase I -
  OH128_RS03465 (OH128_03465) - 689978..690769 (+) 792 WP_031263840.1 3'-5' exonuclease -
  OH128_RS03470 (OH128_03470) galE 690826..691812 (+) 987 WP_264378414.1 UDP-glucose 4-epimerase GalE -
  OH128_RS03475 (OH128_03475) - 691806..693506 (+) 1701 WP_002787738.1 ABC transporter ATP-binding protein -
  OH128_RS03480 (OH128_03480) - 693503..694579 (+) 1077 WP_002787737.1 glycosyltransferase -
  OH128_RS03485 (OH128_03485) - 694572..695501 (+) 930 WP_002777213.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 36184.65 Da        Isoelectric Point: 10.1355

>NTDB_id=749550 OH128_RS03415 WP_052779190.1 679476..680420(-) (waaF) [Campylobacter coli strain CC20JX12]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELDKIDLSFT
FRSAFSSKIILHILKTRQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHSKDLKLPFKLKFQNPIVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKICPLKHHKCMKDLKPEMVLEKLKFDT

Nucleotide


Download         Length: 945 bp        

>NTDB_id=749550 OH128_RS03415 WP_052779190.1 679476..680420(-) (waaF) [Campylobacter coli strain CC20JX12]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCAGTGATGGCTTCGCCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCTCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGTAAAGAACTTGATAAAATCGATCTTAGCTTCACT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTTAAAACAAGACAAAGATATTTTTTTGACAAAAATAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTAGATATCAAAGCTCATTCTAAGGATTTAA
AACTCCCTTTTAAATTAAAATTTCAAAATCCTATTGTTTTAAAAAATGGCAAAAAAATCTTAGGTTTAAACCCTGGAGCA
AGCTTTGGAAGCGCTAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTGA
AAAATCTTTGCAACAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTACACTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAATCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAATGGTTTTAGAAAAATTAAAATTTGATACTTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.248

99.045

0.924