Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FOF60_RS17045 Genome accession   NZ_CP109811
Coordinates   3311965..3313041 (-) Length   358 a.a.
NCBI ID   WP_192470904.1    Uniprot ID   -
Organism   Mesobacillus jeotgali strain SBJS01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306965..3318041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOF60_RS17000 (FOF60_17000) - 3307468..3308256 (+) 789 WP_192470913.1 YqhG family protein -
  FOF60_RS17005 (FOF60_17005) - 3308306..3308488 (-) 183 WP_192470912.1 YqzE family protein -
  FOF60_RS17010 (FOF60_17010) - 3308530..3309030 (-) 501 WP_192470911.1 shikimate kinase -
  FOF60_RS17015 (FOF60_17015) comGG 3309047..3309358 (-) 312 WP_192470910.1 competence type IV pilus minor pilin ComGG -
  FOF60_RS17020 (FOF60_17020) comGF 3309418..3309879 (-) 462 WP_192470909.1 competence type IV pilus minor pilin ComGF -
  FOF60_RS17025 (FOF60_17025) - 3309845..3310165 (-) 321 WP_192470908.1 hypothetical protein -
  FOF60_RS17030 (FOF60_17030) comGD 3310149..3310565 (-) 417 WP_192470907.1 competence type IV pilus minor pilin ComGD -
  FOF60_RS17035 (FOF60_17035) comGC 3310586..3310909 (-) 324 WP_192470906.1 competence type IV pilus major pilin ComGC -
  FOF60_RS17040 (FOF60_17040) comGB 3310947..3311978 (-) 1032 WP_192470905.1 competence type IV pilus assembly protein ComGB -
  FOF60_RS17045 (FOF60_17045) comGA 3311965..3313041 (-) 1077 WP_192470904.1 competence type IV pilus ATPase ComGA Machinery gene
  FOF60_RS17055 (FOF60_17055) - 3313283..3313636 (-) 354 WP_192470903.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FOF60_RS17060 (FOF60_17060) - 3313829..3314530 (+) 702 WP_192470902.1 helix-turn-helix transcriptional regulator -
  FOF60_RS17065 (FOF60_17065) - 3314610..3314855 (+) 246 WP_023627332.1 DUF2626 domain-containing protein -
  FOF60_RS17070 (FOF60_17070) - 3314918..3316009 (-) 1092 WP_192470901.1 class I SAM-dependent methyltransferase -
  FOF60_RS17075 (FOF60_17075) - 3316180..3316815 (-) 636 WP_192470900.1 MBL fold metallo-hydrolase -
  FOF60_RS17080 (FOF60_17080) - 3316983..3317462 (+) 480 WP_192470899.1 hypothetical protein -
  FOF60_RS17085 (FOF60_17085) - 3317575..3317748 (+) 174 WP_192470898.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 40161.25 Da        Isoelectric Point: 8.7803

>NTDB_id=749501 FOF60_RS17045 WP_192470904.1 3311965..3313041(-) (comGA) [Mesobacillus jeotgali strain SBJS01]
MIIVKSIEQLAENVLKDALRSGASDIHIIPREKDTLIKFRLGNQLLPRYTLEQADCERLISHFKFTASMDIGEKRRPQSG
AFTYRYQEMKIGLRISTLPAYQSESMVIRLLPEQNQSPSFQISLFPSTSKKLISLLKHAHGLIIFTGPTGSGKTTTLYSM
LSETSGLVNRNVITLEDPIEKPSDTVLQVQVNERAGISYSAGLKAILRHDPDIIMVGEIRDKETAETAIRASLTGHLVLT
TMHTRDARGAIYRLIEFGINWLEIEQTLIAVTAQRLVELTCPYCEGTCSPFCYSSGSGKRGNIFEILTGKDLSAVLKEAK
GERQNYRYKTLKDAIRKGIALGYIKESEYQRWVLNDGE

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=749501 FOF60_RS17045 WP_192470904.1 3311965..3313041(-) (comGA) [Mesobacillus jeotgali strain SBJS01]
ATGATTATTGTCAAATCAATTGAACAGCTAGCGGAAAATGTCTTGAAAGATGCTTTACGAAGTGGCGCGTCGGATATCCA
TATCATTCCTCGCGAGAAAGACACACTGATTAAATTCAGGCTAGGCAATCAGCTTCTCCCCCGGTATACGCTTGAACAGG
CTGATTGTGAGCGGCTCATTTCCCACTTTAAATTCACAGCTTCGATGGATATTGGTGAAAAAAGACGGCCTCAAAGCGGT
GCTTTTACCTATCGATACCAAGAAATGAAGATTGGCTTAAGGATCTCCACGCTTCCAGCCTACCAGAGCGAAAGCATGGT
CATTCGACTCCTTCCCGAACAAAATCAATCTCCTTCTTTCCAAATTTCATTATTTCCATCCACATCAAAAAAACTGATTT
CCCTTTTAAAACACGCACATGGTTTGATCATTTTTACTGGACCGACGGGCAGCGGCAAGACGACTACCCTTTATTCCATG
CTTTCTGAAACCTCGGGTTTAGTCAATCGCAATGTCATCACCCTTGAAGATCCGATTGAAAAACCGAGCGACACAGTACT
GCAGGTCCAAGTCAATGAACGGGCTGGAATTTCATATTCCGCTGGCTTAAAGGCCATTTTACGGCATGATCCGGATATTA
TCATGGTTGGGGAAATTCGGGATAAAGAAACTGCTGAAACGGCGATAAGAGCTTCGCTGACAGGACATCTCGTCCTGACG
ACGATGCATACAAGGGATGCAAGAGGAGCGATTTACAGGCTGATTGAATTCGGGATTAATTGGCTTGAGATTGAACAGAC
ATTGATTGCAGTAACAGCTCAAAGGCTCGTTGAACTTACATGTCCATATTGTGAAGGGACTTGCTCGCCATTTTGCTATT
CATCAGGCAGCGGCAAAAGAGGAAATATCTTCGAAATCCTTACGGGGAAGGATTTATCCGCTGTTCTGAAGGAAGCAAAG
GGAGAGCGGCAAAACTATCGATACAAGACGCTCAAAGATGCAATCCGGAAAGGAATCGCACTGGGGTACATCAAGGAGTC
AGAGTACCAGAGGTGGGTTCTCAATGATGGAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.899

99.441

0.556
  pilB Glaesserella parasuis strain SC1401

38.682

97.486

0.377