Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QET41_RS02835 Genome accession   NZ_CP123231
Coordinates   587867..589300 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 5132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582867..594300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QET41_RS02800 (QET41_02800) elbB 583558..584211 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  QET41_RS02805 (QET41_02805) mtgA 584208..584936 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QET41_RS02810 (QET41_02810) yrbL 584933..585565 (-) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  QET41_RS02815 (QET41_02815) npr 585779..586051 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QET41_RS02820 (QET41_02820) rapZ 586048..586902 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QET41_RS02825 (QET41_02825) ptsN 586948..587439 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QET41_RS02830 (QET41_02830) hpf 587557..587844 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QET41_RS02835 (QET41_02835) rpoN/rpoN1 587867..589300 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  QET41_RS02840 (QET41_02840) lptB 589348..590073 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QET41_RS02845 (QET41_02845) lptA 590080..590637 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QET41_RS02850 (QET41_02850) lptC 590606..591181 (-) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  QET41_RS02855 (QET41_02855) kdsC 591178..591744 (-) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QET41_RS02860 (QET41_02860) kdsD 591765..592751 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QET41_RS02865 (QET41_02865) yrbG 592765..593742 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=749434 QET41_RS02835 WP_000809051.1 587867..589300(-) (rpoN/rpoN1) [Escherichia coli strain 5132]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=749434 QET41_RS02835 WP_000809051.1 587867..589300(-) (rpoN/rpoN1) [Escherichia coli strain 5132]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465